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Characterization of edwardsiella species isolated from fish by using genomic DNA fingerprinting technique
Joint Authors
Shalabi, A. M.
Anani, M. E.
Nur al-Din, A.I
El Kattawy, Z. M..
Wahdan, A.
Source
Suez Canal Veterinary Medicine Journal
Issue
Vol. 23, Issue 2 (31 Dec. 2018), pp.215-223, 9 p.
Publisher
Suez Canal University Faculty of Veterinary Medicine
Publication Date
2018-12-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Nowadays, Edwardsiella is considered the most dangerous bacterial problem in cultured fish farms.
In this study, 100 diseased Nile tilapia (Oreochromis niloticus) and 100 diseased sea bass (Dicentrarchus labrax) were collected randomly from kafer El-sheikh cultured fish farms and also from Damietta Government during the period from May 2016 till May 2017.
All diseased fishes showed hemorrhages, all over the body surface accompanied by swelling of abdomen were observed clinically and post-mortem.
Liver, kidney and spleen samples were subjected to bacteriological examinations, for isolation of E.
tarda.
The suspected isolates were characterized morphologically and biochemically including microbact 24.
PCR technique was applied for detection of some virulence genes (etfA, etfD, gyerB genes) in E.
tarda isolates.
Total prevalence of well identified E.
tarda isolates was 12% in Oreochromis niloticus while 7% in Dicentrarchus labrax.
Most E.
tarda isolates were sensitive to ciprofloxacin, streptomycin chloramphenicol and gentamycin, conversely, most of isolates were resistant to tetracyclin, nalidixic acid, ampicillin, and sulphamethoxazole.
PCR results recorded that all recovered E.
tarda isolates contain gyerB at 415bp and etfD at 445 bp, while not all E.
tarda posses etfA at 415bp
American Psychological Association (APA)
Anani, M. E.& Shalabi, A. M.& Nur al-Din, A.I& Wahdan, A.& El Kattawy, Z. M... 2018. Characterization of edwardsiella species isolated from fish by using genomic DNA fingerprinting technique. Suez Canal Veterinary Medicine Journal،Vol. 23, no. 2, pp.215-223.
https://search.emarefa.net/detail/BIM-959560
Modern Language Association (MLA)
Anani, M. E.…[et al.]. Characterization of edwardsiella species isolated from fish by using genomic DNA fingerprinting technique. Suez Canal Veterinary Medicine Journal Vol. 23, no. 2 (2018), pp.215-223.
https://search.emarefa.net/detail/BIM-959560
American Medical Association (AMA)
Anani, M. E.& Shalabi, A. M.& Nur al-Din, A.I& Wahdan, A.& El Kattawy, Z. M... Characterization of edwardsiella species isolated from fish by using genomic DNA fingerprinting technique. Suez Canal Veterinary Medicine Journal. 2018. Vol. 23, no. 2, pp.215-223.
https://search.emarefa.net/detail/BIM-959560
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 221-223
Record ID
BIM-959560