Multiplex PCR of some antibiotic resistance genes of methicillin resistant staphylococcus aureus (MRSA)‎ isolated from infected cat fish (Clarias garipeneaus)‎

Abstract EN

This study was designed to detect some antibiotic resistance genes of S.aureus by using PCR.

A total of 150 Cat fish (Clarias garipeneaus) with a body weight ranged from200 – 250g.

suffered from ulcerated skin and erosion.

S.

aureus were isolated in percent of 100% from infected fish by using Baird parker as selective media.

By using multiplex PCR for investigation some antibiotic resistant genes, as erm A gen, aac (6)-aph (2) genes , van A gen and mec A gene.

All isolated S.

aureus were sensitive to Amikacin, Amoxicillin, Ampicillin and Ciprofloxacin while resistant to Gentamicin, Methicillin and Neomycin