Detection of intracellular adhesion (ICA)‎ B gene in staphylococcus aureus isolated from wound infections

Abstract EN

Background:Staphylococcus aureus is an imperative cause of community acquired infections and nosocomial infections,The formation of biofilm on host surfaces and adherence is painstaking to be significant virulence factor in S.

aureus.

Objective:The main objective of this study is to detect and describe the distribution of S.

aureus and icaB gene in wound infection samples taken from patients at Al-Hussein Teaching Hospital in Thi-Qar province, Iraq, during the period extended from February to September 2007.

Materials and Methods:A total number of bacterial isolatesincluded 130 sample recovered from different woundsof patients in burn unit.

All isolates of the targeted bacteria were subjected for screening the biofilm allied gene (icaB) by Polymerase Chain Reaction (PCR) technique.

Results: The recent data indicated that 72 (55.3%) identified as S.

aureus, and the females were likely more affected with S.

aureus infection than males (47 > 25).

The results of PCR recorded that icaB gene was expressedonly in 61% of all isolates.

Conclusion: The recent results recorded a high percentage of icaB gene which related with biofilm production may be increased the pathogenicity of this pathogen which caused different human diseases.