The use of different stabilizers for improving integrity of the locally prepared lyophilized brucella melitensis rev 1 vaccine
Joint Authors
Husayn, H. I.
Ghazi, Nabilah A.
Abd al-Aziz, Wafa R.
Shell, W. S.
Ibrahim, H. M.
Source
Journal of Veterinary Medical Research
Issue
Vol. 25, Issue 1 (30 Jun. 2018), pp.59-67, 9 p.
Publisher
Beni-Suef University Faculty of Veterinary Medicine
Publication Date
2018-06-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Stability study of biological products especially living bacterial vaccines plays an important role for determination of product changes in maintenance period and ensures safety, efficacy and maintenance of biological properties of the vaccines.
so, the objective of this study was to establish stability and keeping quality of the local Brucella melitensis Rev-1 vaccine using different types of stabilizers in lyophilization process.
a long-term stability study was carried out for four batches of reduced-dose brucella melitensis Rev-1 vaccine manufactured by veterinary serum and vaccine research institute using four different stabilizers.
these stabilizers were (A) sucrose and skimmed milk, (B and C) different concentrations of sucrose, sodium glutamate and gelatin, and (D) casein, sucrose and sodium glutamate.
the quality control tests including colony forming unit, purity, dissociation and physicochemical tests on all batches until 12 months post- production were performed.
the obtained results indicated that, in spite of collapse (shrinkage) of lyophilized cake in a number of bottles in batches prepared using stabilizer A, the brucella vaccine batches were stable and met the specification recommended by OIE 2012 for 12 months post-production in vaccine batches with stabilizers A and D.
American Psychological Association (APA)
Ghazi, Nabilah A.& Abd al-Aziz, Wafa R.& Ibrahim, H. M.& Shell, W. S.& Husayn, H. I.. 2018. The use of different stabilizers for improving integrity of the locally prepared lyophilized brucella melitensis rev 1 vaccine. Journal of Veterinary Medical Research،Vol. 25, no. 1, pp.59-67.
https://search.emarefa.net/detail/BIM-967552
Modern Language Association (MLA)
Ghazi, Nabilah A.…[et al.]. The use of different stabilizers for improving integrity of the locally prepared lyophilized brucella melitensis rev 1 vaccine. Journal of Veterinary Medical Research Vol. 25, no. 1 (2018), pp.59-67.
https://search.emarefa.net/detail/BIM-967552
American Medical Association (AMA)
Ghazi, Nabilah A.& Abd al-Aziz, Wafa R.& Ibrahim, H. M.& Shell, W. S.& Husayn, H. I.. The use of different stabilizers for improving integrity of the locally prepared lyophilized brucella melitensis rev 1 vaccine. Journal of Veterinary Medical Research. 2018. Vol. 25, no. 1, pp.59-67.
https://search.emarefa.net/detail/BIM-967552
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-967552