Genetic analysis of multidrug resistant salmonella isolated from broiler chickens

Abstract EN

Salmonellosis is a major problem for the poultry industry, and this problem represents a critical food safety hazard.

resistance to antimicrobial agents within nontyphoidal salmonellae is a serious problem.

the present study aimed to analyze genetically some B-lactamase resistance genes and some virulence associated genes in salmonella isolates from broiler chicken.

five hundred samples were collected from diseased broiler chickens of different ages (3-6 weeks) from different farms in Assiut governorate in Egypt during the period from January 2015 to December 2015.

bacteriological examination showed that 26 salmonella isolates were recovered with a prevalence rate of 5.2% serotyping of salmonella isolates showed that s.

enteritidis s.

typhimurium, and s.

kentuky were identified at rates of 50%, 30.8% and 19.2%, respectively.

results of antibiogram showed that 18 salmonella isolates (92.3%) were multidrug resistant.

all isolates were screened for the presence of 2 B-lactamase resistance genes (blactx and blacmy) as well as 3 virulence genes (stn, inva and hila) using multiplex pcr.

the overall prevalences were 53.9% for blactx and 34.6% for blacmy.

meanwhile, stn, inva and hila genes were found in 96.2%, 100% and 84.7% of isolates, respectively.