In vitro regeneration of Pimpinella Anisum L. using different plant growth regulators
Other Title(s)
إكثار نبات .Pimpinella Anisum L خارج الجسم الحي باستعمال منظمات نمو نباتية مختلفة
Joint Authors
Abd al-Halim, Ansam Ghazi
Ghani, Fatimah Haydar
Muhsin, Nura Muhammad
Source
Issue
Vol. 60, Issue 4 (30 Apr. 2019), pp.715-723, 9 p.
Publisher
University of Baghdad College of Science
Publication Date
2019-04-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract EN
Plant regeneration protocols were developed for medicinally important anise (Pimpinella anisum L.) that successfully achieved from seeds.
Seeds were sterilized and inoculated on Murashige and Skoog (MS) medium with and without gibberellins (GA3) until full germination.
The highest percentage of germination (100%) was recorded on MS medium treated with 2.0 mg/L GA3 after 7 days.
For shoot proliferation, different concentrations of 6- benzyl adenine BA (1, 1.5, 2 mg/L) were used.
To enhance shoot induction, 0.1 mg/L of naphthalene acetic acid (NAA) and 0.01 mg/L of thidiazuron (TDZ) were tested along with BA.
Direct regenerated shoots were obtained on MS medium supplemented with BA alone (2mg/L) which gave (7shoot/explant), while the presence of NAA and TDZ at low concentration encourage of somatic embryogenesis.
A single stem explant was able to produce 25 micro shoots after (24) days of culture.
Rooting was experimented at different levels of NAA (0, 0.5, 1, 1.5 mg/L).
The medium that devoided from growth regulator and the media with the lowest level of NAA were the most effective in inducing root.
Finally, it was concluded that cytokinins are essential for inducing the direct regeneration and the addition of auxin along with there is necessary for the induction of somatic embryogenesis.
American Psychological Association (APA)
Abd al-Halim, Ansam Ghazi& Ghani, Fatimah Haydar& Muhsin, Nura Muhammad. 2019. In vitro regeneration of Pimpinella Anisum L. using different plant growth regulators. Iraqi Journal of Science،Vol. 60, no. 4, pp.715-723.
https://search.emarefa.net/detail/BIM-969245
Modern Language Association (MLA)
Abd al-Halim, Ansam Ghazi…[et al.]. In vitro regeneration of Pimpinella Anisum L. using different plant growth regulators. Iraqi Journal of Science Vol. 60, no. 4 (2019), pp.715-723.
https://search.emarefa.net/detail/BIM-969245
American Medical Association (AMA)
Abd al-Halim, Ansam Ghazi& Ghani, Fatimah Haydar& Muhsin, Nura Muhammad. In vitro regeneration of Pimpinella Anisum L. using different plant growth regulators. Iraqi Journal of Science. 2019. Vol. 60, no. 4, pp.715-723.
https://search.emarefa.net/detail/BIM-969245
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 721-723
Record ID
BIM-969245