Development of hydrogen peroxide biosensor based on immobilization of hemoglobin on screen-printed carbon electrode modified with silver nanoparticles
Other Title(s)
تطوير متحسس حيوي لبيروكسيد الهيدروجين باستخدام الهيموغلوبين المقيد على قطب الكاربون المطبوع على شريحة و المحور بدقائق الفضة النانوية
Joint Authors
Abd al-Rahman, Shadha Abd al-Wadud
al-Samirrai, Abd al-Karim Muhammad Ali
Ulaywi, Ali Sad
Source
Issue
Vol. 60, Issue 11 (30 Nov. 2019), pp.2332-2340, 9 p.
Publisher
University of Baghdad College of Science
Publication Date
2019-11-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract EN
The direct electron transfer behavior of hemoglobin that is immobilized onto screen-printed carbon electrode (SPCE) modified with silver nanoparticles (AgNPs) and chitosan (CS) was studied in this work.
Cyclic voltametry and spectrophotometry were used to characterize the hemoglobin (Hb) bioconjunction with AgNPs and CS.
Results of the modified electrode showed quasi-reversible redox peaks with a formal potential of (-0.245V) versus Ag/AgCl in 0.1M phosphate buffer solution (PBS), pH7, at a scan rate of 0.1Vs-1.
The charge transfer coefficient (α) was 0.48 and the apparent electron transfer rate constant (Ks) was 0.47s-1.
The electrode was used as a hydrogen peroxide biosensor with a linear response over 3 to 240 µM and a detection limit of 0.6 µM.
As a result, the modified biosensor here has exhibited a high sensitivity, good reproducibility and stability
American Psychological Association (APA)
Ulaywi, Ali Sad& Abd al-Rahman, Shadha Abd al-Wadud& al-Samirrai, Abd al-Karim Muhammad Ali. 2019. Development of hydrogen peroxide biosensor based on immobilization of hemoglobin on screen-printed carbon electrode modified with silver nanoparticles. Iraqi Journal of Science،Vol. 60, no. 11, pp.2332-2340.
https://search.emarefa.net/detail/BIM-969388
Modern Language Association (MLA)
Ulaywi, Ali Sad…[et al.]. Development of hydrogen peroxide biosensor based on immobilization of hemoglobin on screen-printed carbon electrode modified with silver nanoparticles. Iraqi Journal of Science Vol. 60, no. 11 (2019), pp.2332-2340.
https://search.emarefa.net/detail/BIM-969388
American Medical Association (AMA)
Ulaywi, Ali Sad& Abd al-Rahman, Shadha Abd al-Wadud& al-Samirrai, Abd al-Karim Muhammad Ali. Development of hydrogen peroxide biosensor based on immobilization of hemoglobin on screen-printed carbon electrode modified with silver nanoparticles. Iraqi Journal of Science. 2019. Vol. 60, no. 11, pp.2332-2340.
https://search.emarefa.net/detail/BIM-969388
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-969388