Analysis of morphine, morphine-3-glucuronide, morphine-6-glucuronide, codeine and codeine-6-glucuronide using HPLC

Other Title(s)

تحليل المورفين، المورفين -3 غلوكورونيد، المورفين -6-غلوكورونيد، الكودايين و الكودايين-6-الغلوكورونيد باستخدام جهاز الفصل السائل عالي الكفاءة

Joint Authors

al-Mutayri, Abd al-Latif Ayid
al-Shuqayr, Muhammad Sulayman
Bin Baz, Abd al-Majid Sad
al-Anqari, Walid Abd al-Aziz
al-Sakran, Sultan Hamdan
al-Mutayri, Turki Ayid
al-Bulayhi, Ahmad Abd al-Aziz
al-Anzi, Muhammad Musir

Source

Journal of Medical and Pharmaceutical Sciences

Issue

Vol. 4, Issue 1 (31 Mar. 2020), pp.83-110, 28 p.

Publisher

National Research Center

Publication Date

2020-03-31

Country of Publication

Palestine (Gaza Strip)

No. of Pages

28

Main Topic

Pharmacology

Topics

Abstract EN

In interpretive forensic and clinical toxicology, there is a great need for the identification and detection of opiate drugs and their various glucuronide metabolites.

Glucuronides are usually determined by cleavage of the glucuronide bond with an enzyme (e.g., β-glucuronidase) or acid hydrolysis to yield the parent compound, which is subsequently detected.

For Gas chromatography-mass spectrometry (GC-MS) analysis this may be via derivatization to a more volatile or stable form.

Direct detection of the glucuronide conjugates overcomes the critical limitations of these approaches.

In this conduct, a rapid and selective reversed-phase high-performance liquid chromatographic (HPLC) assay with gradient elution and ultraviolet detection for morphine, morphine-3-glucuronide, morphine-6-glucuronide, codeine, codeine-6-glucuronide and hydrocodone was developed.

The separation was performed on a gemini C18 (Octadecyl carbon chain) analytical column (150  2.0 mm, 5 µm) and detected by an ultraviolet (UV) detector at 210 nm.

The mobile phase consisted of an acetonitrile–phosphate buffer (0.0125 M, pH 7.5) with elution in the gradient mode ranging from 7.5–60% acetonitrile over 25 minutes (min) with a 0.2 mL/min flow rate.

The calibration curves were linear (R2 range 0.997–0.999) in the concentration range 0.025–2 µg/mL for all analytes, using hydrocodone as the internal standard.

Therefore, this conduct aims to develop an efficient method of separation of morphine, codeine and glucuronides by HPLC-UV and to evaluate the use of SPME LC tips for the extraction of morphine, codeine and glucuronides from urine samples

American Psychological Association (APA)

al-Mutayri, Abd al-Latif Ayid& al-Shuqayr, Muhammad Sulayman& Bin Baz, Abd al-Majid Sad& al-Anqari, Walid Abd al-Aziz& al-Sakran, Sultan Hamdan& al-Mutayri, Turki Ayid…[et al.]. 2020. Analysis of morphine, morphine-3-glucuronide, morphine-6-glucuronide, codeine and codeine-6-glucuronide using HPLC. Journal of Medical and Pharmaceutical Sciences،Vol. 4, no. 1, pp.83-110.
https://search.emarefa.net/detail/BIM-969630

Modern Language Association (MLA)

al-Mutayri, Abd al-Latif Ayid…[et al.]. Analysis of morphine, morphine-3-glucuronide, morphine-6-glucuronide, codeine and codeine-6-glucuronide using HPLC. Journal of Medical and Pharmaceutical Sciences Vol. 4, no. 1 (Mar. 2020), pp.83-110.
https://search.emarefa.net/detail/BIM-969630

American Medical Association (AMA)

al-Mutayri, Abd al-Latif Ayid& al-Shuqayr, Muhammad Sulayman& Bin Baz, Abd al-Majid Sad& al-Anqari, Walid Abd al-Aziz& al-Sakran, Sultan Hamdan& al-Mutayri, Turki Ayid…[et al.]. Analysis of morphine, morphine-3-glucuronide, morphine-6-glucuronide, codeine and codeine-6-glucuronide using HPLC. Journal of Medical and Pharmaceutical Sciences. 2020. Vol. 4, no. 1, pp.83-110.
https://search.emarefa.net/detail/BIM-969630

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 107-110

Record ID

BIM-969630