Assessment of genotoxicity of Citrobacter freundii bacteriocin on bone marrow cells in albino mice
Other Title(s)
تقييم السمية الجينية لبكتريوسين Citrobacter freundii خلايا نخاع عظم الفئران البيض
Joint Authors
Hammad, Rasha Nuri
Ubayd, Hind Husayn
Source
Issue
Vol. 61, Issue 5 (31 May. 2020), pp.999-1007, 9 p.
Publisher
University of Baghdad College of Science
Publication Date
2020-05-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Topics
Abstract EN
Genotoxic effects of crude bacteriocin extracted from Citrobacter freundii were detected on albino mice bone marrow cells in vivo, using micronucleus (MN) and comet assay.
The mice were administered intraperitoneally with 37.5, 75, 150 and 300 mg/kg of the extract for 24 hours.
C.
freundii was isolated from patients suffering from urinary tract infections (UTI).
The bacteriocin producing isolates were determined using cup assayand the most efficient bacteriocin producers were selected.
Bacteriocin was extracted from the efficient isolates via the induction with Mitomycin-C (2 mg/ml).
Bacteriocin activity (320 U/ml) was determined by well diffusion method, while the protein concentration (2900µg/ml) was estimated by Lowery method.
The results showed an acute dose-dependent toxic effect of the crude bacteriocin ; The higher doses (150 and 300 mg/kg) caused a significant increase (P≤0.05) in the micronuclei frequency in the bone marrow cells (4.62 and 5.37%, respectively (.
Furthermore, DNA damage increased significantly (P≤0.05) and proportionally to higher bacteriocin doses (75, 150 and 300 mg/kg), as demonstrated by increased values of tail length (145.18, 267.73 and 295.08 %,( %DNA in tail (8.05, 13.87 and 14.31 %(, and olive tail moment (13.25, 22.72 and 25.85 % , respectively.
American Psychological Association (APA)
Hammad, Rasha Nuri& Ubayd, Hind Husayn. 2020. Assessment of genotoxicity of Citrobacter freundii bacteriocin on bone marrow cells in albino mice. Iraqi Journal of Science،Vol. 61, no. 5, pp.999-1007.
https://search.emarefa.net/detail/BIM-970209
Modern Language Association (MLA)
Hammad, Rasha Nuri& Ubayd, Hind Husayn. Assessment of genotoxicity of Citrobacter freundii bacteriocin on bone marrow cells in albino mice. Iraqi Journal of Science Vol. 61, no. 5 (2020), pp.999-1007.
https://search.emarefa.net/detail/BIM-970209
American Medical Association (AMA)
Hammad, Rasha Nuri& Ubayd, Hind Husayn. Assessment of genotoxicity of Citrobacter freundii bacteriocin on bone marrow cells in albino mice. Iraqi Journal of Science. 2020. Vol. 61, no. 5, pp.999-1007.
https://search.emarefa.net/detail/BIM-970209
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-970209