Immunohistochemical expression of P16 protein and TGF β1 in mice liver exposed to Fumonisin B1

Other Title(s)

التعبير المناعي النسيجي للبروتين ب 16 و عامل النمو المحول بيتا 1 في كبد الفئران المعرضة للسم فيومنسين ب 1

Joint Authors

Ismail, Munirah Julub
Muhammad, Sinai Walid
Khalaf, Nur al-Huda Fakhri
Khashman, Basim Muhammad
al-Maliki, Maysa Kazim Habash

Source

Baghdad Science Journal

Issue

Vol. 17, Issue 2 (30 Jun. 2020), pp.401-405, 5 p.

Publisher

University of Baghdad College of Science for Women

Publication Date

2020-06-30

Country of Publication

Iraq

No. of Pages

5

Main Topic

Sports

Abstract EN

Fumonisin B1 (FB1) is a mycotoxin produced in some grains (mainly corn) by Fusarium species.

Due to a structural similarity between FB1 and sphinganine, sphingolipids metabolism is inhibited.

Such inhibition plays a critical role in cell to cell singling and structure of lipoprotein; therefore FB1 has been suggested to have a relationship with human and animal cancer.

This research is planned to study the effect of FB1 on male mice at two doses (20 and 30 μg/ ml) on the expression of TGF-β1 and p16 in liver cells.

Three groups of Swiss albino male mice; each group was orally administrated with FB1 toxin as the following: normal saline (control group); 20 and 30 μg/ ml.

All groups were sacrificed after two weeks of oral management.

Liver samples were collected and prepared for immunohistochemistry technique (IHC) using anti-TGF-β1 and anti-p16 antibodies.

The results showed that exposure to FB1 caused significant elevation of TGF-β1 in both doses (76.74 ± 2.387% and 80.62 ± 7.277%, respectively) in comparison with the control group (46.79 ± 2.404%).

The level of p16 protein was decreased at 20 μg/ml (76.63 ± 2.349%) and then increased at 30 μg/ml (81.25 ± 6.263%) but the expression was lower than that of control (90.00 ± 0.805%).

In conclusion, FB1 has a significant effect on TGF-β1 and p16 protein expression at both doses (20 and 30 μg/ml), and therefore, its role in cancer development is suggested.

American Psychological Association (APA)

Muhammad, Sinai Walid& Khashman, Basim Muhammad& Khalaf, Nur al-Huda Fakhri& Ismail, Munirah Julub& al-Maliki, Maysa Kazim Habash. 2020. Immunohistochemical expression of P16 protein and TGF β1 in mice liver exposed to Fumonisin B1. Baghdad Science Journal،Vol. 17, no. 2, pp.401-405.
https://search.emarefa.net/detail/BIM-970232

Modern Language Association (MLA)

Muhammad, Sinai Walid…[et al.]. Immunohistochemical expression of P16 protein and TGF β1 in mice liver exposed to Fumonisin B1. Baghdad Science Journal Vol. 17, no. 2 (2020), pp.401-405.
https://search.emarefa.net/detail/BIM-970232

American Medical Association (AMA)

Muhammad, Sinai Walid& Khashman, Basim Muhammad& Khalaf, Nur al-Huda Fakhri& Ismail, Munirah Julub& al-Maliki, Maysa Kazim Habash. Immunohistochemical expression of P16 protein and TGF β1 in mice liver exposed to Fumonisin B1. Baghdad Science Journal. 2020. Vol. 17, no. 2, pp.401-405.
https://search.emarefa.net/detail/BIM-970232

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 403-404

Record ID

BIM-970232