Prevalence of quinolones resistance proteins encoding genes (qnr genes) and Co-resistance with β-lactams among Klebsiella pneumoniae isolates from Iraqi patients
Other Title(s)
التحري عن الجينات المشفرة لبروتينات مقاومة الكينولينات (جينات qnr) و المقاومة المشتركة مع البيتالاكتام في عزلات Klebsiella pneumoniae من المرضى العراقيين
Joint Authors
Mustafa, Mustafa Suhayl
Abd Allah, Rana Mujahid
Source
Issue
Vol. 17, Issue 2 (30 Jun. 2020), pp.406-414, 9 p.
Publisher
University of Baghdad College of Science for Women
Publication Date
2020-06-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract EN
This study investigated the prevalence of quinolones resistance proteins encoding genes (qnr genes) and co-resistance for fluoroquinolones and β-lactams among clinical isolates of Klebsiella pneumoniae.
Out of 150 clinical samples, 50 isolates of K.
pneumoniae were identified according to morphological and biochemical properties.
These isolates were collected from different clinical samples, including 15 (30%) urine, 12 (24%) blood, 9 (18%) sputum, 9 (18%) wound, and 5 (10%) burn.
The minimum inhibitory concentrations (MICs) assay revealed that 15 (30%) of isolates were resistant to ciprofloxacin (≥4μg/ml), 11 (22%) of isolates were resistant to levofloxacin (≥8 μg/ml), 21 (42%) of isolates were resistant to ertapenem (≥8 μg/ml), 18 (36%) of isolates were resistant to imipenem (4- ≥16μg/ml), 43 (86%) of isolates were resistant to ceftriaxone (4- ≥64 μg/ml), 42 (84%) of isolates were resistant to ceftazidime (16-64 μg/ml), and 40 (80%) of isolates were resistant to cefepime (4- ≥16μg/ml).
The results revealed that all fluoroquinolone resistant K.
pneumoniae isolates were resistant for β-lactams that used in this study.
Genotypic detection of qnr genes revealed that qnrS and qnrB were found in 38 (76%) and 18 (36%) of K.
pneumoniae isolates, respectively.
On the other hand, qnrA, qnrC, and qnrD were not found among K.
pneumoniae isolates.
DNA sequencing of qnrB gene revealed that the presence of silent and missense mutations that may have led to increase the resistance values of MICs for ciprofloxacin and levofloxacin.
These variants were registered in NCBI at the accession numbers LC373260 and LC381730.
The phylogenetic tree of qnrB variants showed a significant deviation of these variants from K.
pneumoniae species.
The spread of qnr genes among clinical isolates of K.
pneumoniae and high association observed between resistance to fluoroquinolones and β-lactams have led to a major threat to public health through development of MDR K.
pneumoniae.
American Psychological Association (APA)
Mustafa, Mustafa Suhayl& Abd Allah, Rana Mujahid. 2020. Prevalence of quinolones resistance proteins encoding genes (qnr genes) and Co-resistance with β-lactams among Klebsiella pneumoniae isolates from Iraqi patients. Baghdad Science Journal،Vol. 17, no. 2, pp.406-414.
https://search.emarefa.net/detail/BIM-970233
Modern Language Association (MLA)
Mustafa, Mustafa Suhayl& Abd Allah, Rana Mujahid. Prevalence of quinolones resistance proteins encoding genes (qnr genes) and Co-resistance with β-lactams among Klebsiella pneumoniae isolates from Iraqi patients. Baghdad Science Journal Vol. 17, no. 2 (2020), pp.406-414.
https://search.emarefa.net/detail/BIM-970233
American Medical Association (AMA)
Mustafa, Mustafa Suhayl& Abd Allah, Rana Mujahid. Prevalence of quinolones resistance proteins encoding genes (qnr genes) and Co-resistance with β-lactams among Klebsiella pneumoniae isolates from Iraqi patients. Baghdad Science Journal. 2020. Vol. 17, no. 2, pp.406-414.
https://search.emarefa.net/detail/BIM-970233
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 413-414
Record ID
BIM-970233