Molecular analysis of rifampicin resistance conferring mutations in mycobacterium tuberculosis

Other Title(s)

التحليل الجزيئي لمقاومة الريفامبيسين يمنح طفرات في مرض السل المتفطرات

Joint Authors

Tawgozy, Fayruz Hasan Abd Allah
Qarasnji, Bushra Karim

Source

Baghdad Science Journal

Issue

Vol. 17, Issue 2 (30 Jun. 2020), pp.444-451, 8 p.

Publisher

University of Baghdad College of Science for Women

Publication Date

2020-06-30

Country of Publication

Iraq

No. of Pages

8

Main Subjects

Biology
Medicine

Topics

Abstract EN

Mycobacterium tuberculosis resistance to rifampicin is mainly mediated through mutations in the rpoB gene.

The effects of rpoB mutations are relieved by secondary mutations in rpoA or rpoC genes.

This study aims to identify mutations in rpoB, rpoA, and rpoC genes of Mycobacterium tuberculosis isolates and clarify their contribution to rifampicin resistance.

Seventy isolates were identified by acid-fast bacilli smear, Genexpert assay, and growth on Lowenstein Jensen medium.

Drug susceptibility, testing was performed by the proportional method.

DNA extraction, PCR, and sequencing were accomplished for the entire rpoA, rpoB, and rpoC genes.

Twenty-three isolates (32.85%) showed resistance to rifampicin by either proportion method or Genexpert assay.

Sequence analysis of the rpoB gene revealed fourteen different mutation patterns.

Inside the rifampicin resistance determining region (RRDR), codons: S531L, D516V were highly mutated with frequencies of (21.73%, 17.39%) respectively.

Outside the RRDR, there were nine different types of mutations, and M479L was the most prevalent one.

Out of 23 RIF resistant isolates, seven isolates (30.43%) carried mutations in the rpoA gene, and twelve isolates (52.17%) harbored a mutation in rpoC.

Most of the mutations were identified for the first time in this study.

The current study demonstrated that mutations in rpoB, rpoA, and rpoC contributed to RIF resistance in Mycobacterium tuberculosis and this new finding may be relevant to realize how compensatory mutations in the rpoA and rpoC genes restore the fitness cost caused by rifampin resistance-conferring mutations in rpoB.

American Psychological Association (APA)

Tawgozy, Fayruz Hasan Abd Allah& Qarasnji, Bushra Karim. 2020. Molecular analysis of rifampicin resistance conferring mutations in mycobacterium tuberculosis. Baghdad Science Journal،Vol. 17, no. 2, pp.444-451.
https://search.emarefa.net/detail/BIM-970238

Modern Language Association (MLA)

Tawgozy, Fayruz Hasan Abd Allah& Qarasnji, Bushra Karim. Molecular analysis of rifampicin resistance conferring mutations in mycobacterium tuberculosis. Baghdad Science Journal Vol. 17, no. 2 (2020), pp.444-451.
https://search.emarefa.net/detail/BIM-970238

American Medical Association (AMA)

Tawgozy, Fayruz Hasan Abd Allah& Qarasnji, Bushra Karim. Molecular analysis of rifampicin resistance conferring mutations in mycobacterium tuberculosis. Baghdad Science Journal. 2020. Vol. 17, no. 2, pp.444-451.
https://search.emarefa.net/detail/BIM-970238

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 450-451

Record ID

BIM-970238