Five diatom species identified by using potential application of next generation DNA sequencing
Other Title(s)
تشخيص خمسة أنواع دايتومية باستعمال التطبيقات الممكنة لتسلسل الحمض النووي البيئي من الجيل التالي
Joint Authors
al-Mashhadani, Warqa Yahya
Hasan, Fikrat Majid
Source
Bulletin of the Iraq Natural History Museum
Issue
Vol. 16, Issue 1 (31 Mar. 2020), pp.39-61, 23 p.
Publisher
University of Baghdad Iraq Natural History Research Center and Museum
Publication Date
2020-03-31
Country of Publication
Iraq
No. of Pages
23
Main Subjects
Topics
Abstract EN
Molecular barcoding was widely recognized as a powerful tool for the identification of organisms during the past decade; the aim of this study is to use the molecular approach to identify the diatoms by using the environmental DNA.
The diatom specimens were taken from Tigris River.
The environmental DNA(e DNA) extraction and analysis of sequences using the Next Generation Sequencing (NGS) method showed the highest percentage of epipelic diatom genera including Achnanthidium minutissimum (Kützing) Czarnecki, 1994 (21.1%), Cocconeis placentula Ehrenberg, 1838 (21.3 % ) and Nitzschia palea (Kützing) W.
Smith, 1856 (16.3%).
Five species of diatoms: Achnanthidium minutissimum; Fistulifera saprophila (Lange-Bertalot & Bonik) Lange-Bertalot, 1997; Gomphonema pumilum (Grunow) E.
Reichardt & Lange-Bertalot, 1991; Navicula veneta Kützing, 1844 and Thalassiosira pseudonana Hasle Heimdal, 1970 were registered in NCBI under the accession numbers as follows: MN749640.1, MN749641.1, MN749642.1, MN749643.1 and MN749646.1 for the first time; while the two algae Fistulifera saprophila and Thalassiosira pseudonana are regarded as a new record to algal flora in Iraq.
The environmental DNA study will be a catalyst for new studies of biodiversity and environmental studies in Iraq and the region.
American Psychological Association (APA)
al-Mashhadani, Warqa Yahya& Hasan, Fikrat Majid. 2020. Five diatom species identified by using potential application of next generation DNA sequencing. Bulletin of the Iraq Natural History Museum،Vol. 16, no. 1, pp.39-61.
https://search.emarefa.net/detail/BIM-970345
Modern Language Association (MLA)
al-Mashhadani, Warqa Yahya& Hasan, Fikrat Majid. Five diatom species identified by using potential application of next generation DNA sequencing. Bulletin of the Iraq Natural History Museum Vol. 16, no. 1 (2020), pp.39-61.
https://search.emarefa.net/detail/BIM-970345
American Medical Association (AMA)
al-Mashhadani, Warqa Yahya& Hasan, Fikrat Majid. Five diatom species identified by using potential application of next generation DNA sequencing. Bulletin of the Iraq Natural History Museum. 2020. Vol. 16, no. 1, pp.39-61.
https://search.emarefa.net/detail/BIM-970345
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 56-59
Record ID
BIM-970345