Determination of vitamin B6 (pyridoxine hydrochloride) in pharmaceutical preparations using high performance liquid chromatography
Other Title(s)
تقدير فيتامين B6 (هيدروكلوريد البيريدوكسين) بتقنية كروماتوغافيا السائل عالية الأداء
Joint Authors
Mahmud, Hana Shukr
Ahmad, Rabah Riyad
Source
Issue
Vol. 60, Issue 5 (31 May. 2019), pp.943-951, 9 p.
Publisher
University of Baghdad College of Science
Publication Date
2019-05-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Topics
Abstract EN
Determination of vitamin B6 (pyridoxine hydrochloride) was described using high performance liquid chromatographic method.
The analysis was achieved by cosmos IL 5C18-MS-II column (250 mm x 4.6 mm i.
d., 5µm particle size) at room temperature.
The mobile phase used was Acetonitrile, buffer solution (Citric acid, Na2HPO4 pH4) buffer solution in the ratio (70:30) (V: V).
the flow rate was set to 1.25 mL.min-1 and the retention time 1.82 min with UV-detection at 282 nm.
Beer's law was obeyed over the concentration range 10-1250 µg.mL-1.
The method was accurate (relative error % less than 0.05%), precise (RSD better than ±1.05%), average recovery 100.05%, with a limit of detection and quantification of 2.2μg.ml−1, and 7.34μg.mL−1 respectively.
The proposed method was successfully applied to determine the pyridoxine hydrochloride in pharmaceutical preparations in both forms of tablet and injection
American Psychological Association (APA)
Mahmud, Hana Shukr& Ahmad, Rabah Riyad. 2019. Determination of vitamin B6 (pyridoxine hydrochloride) in pharmaceutical preparations using high performance liquid chromatography. Iraqi Journal of Science،Vol. 60, no. 5, pp.943-951.
https://search.emarefa.net/detail/BIM-970626
Modern Language Association (MLA)
Mahmud, Hana Shukr& Ahmad, Rabah Riyad. Determination of vitamin B6 (pyridoxine hydrochloride) in pharmaceutical preparations using high performance liquid chromatography. Iraqi Journal of Science Vol. 60, no. 5 (2019), pp.943-951.
https://search.emarefa.net/detail/BIM-970626
American Medical Association (AMA)
Mahmud, Hana Shukr& Ahmad, Rabah Riyad. Determination of vitamin B6 (pyridoxine hydrochloride) in pharmaceutical preparations using high performance liquid chromatography. Iraqi Journal of Science. 2019. Vol. 60, no. 5, pp.943-951.
https://search.emarefa.net/detail/BIM-970626
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 950-951
Record ID
BIM-970626