Association of catechol-O-methyltransferase gene polymorphism with benign prostatic hyperplasia in Babylon Province

Abstract EN

Background : Benign prostatic hyperplasia (BPH) is a common nonmalignant disorder in elderly men.

Objectives : The objective of the present study was planned to evaluate the frequency and association of catechol-O-methyltransferase (COMT) gene G↔A (Val 158 Met)single-nucleotide polymorphism (SNP) with BPH in Babylon Province.

Materials and Methods : To accomplish this purpose, 146 patients with BPH and 102 apparently healthy controls were subjected to the study.

DNA was extracted from whole blood for all samples.

Genotyping of COMT gene G↔A (Val 158 Met) SNP was carried out by allele specific oligonucleotides polymerase chain reaction.

Results: Results indicated that the homozygous genotype (Met158Met) (AA) of COMT gene G↔A (Val 158 Met) SNP was found to be significantly increase the risk of BPH by three folds with respect to those of the wild genotype (Val158Val) (GG) of COMT gene G↔A (Val 158 Met) SNP.

The heterozygous genotype (Val158Met) (GA) of COMT gene G↔A (Val 158 Met) SNP was found to be none significantly increase the risk of BPH with respect to those of the wild genotype (Val158Val) (GG) of COMT gene G↔A (Val 158 Met) SNP.

The minor allele frequencies (A) of COMT gene G↔A (Val 158 Met) SNP were significantly higher in BPH patients when compared with that of the control group.

Conclusions: The COMT gene G↔A (Val 158 Met) SNP is involved in the pathogenesis of BPH