Spectrophotometric method for the estimation of ceftriaxone in pure form and pharmaceuticals

Abstract EN

Ceftriaxone sodium were one of the widely antibacterial drugs used.

Azo dye derivatization ofdiazonium salt that formed via the reaction between ceftriaxone with hydrochloric acid and sodiumnitrite was developed for the on-research drug analysis then coupling with each one 2, 5-dimethylphenol (2, 5-DMP) and 4-tertbutylphenol (4-TBP) respectively in the alkaline media.

Thedeveloped diazonium coupling methods include an optimization study.

The results show a limit ofdetection and limit of quantification 0.

482, 0.

284 μg/mL, and 1.

607, 0.

945 μg/mL using 2, 5-DMPand 4-TBP reagents respectively.

Moreover, the recovery % obtained was 100.

89%, and 103.

37% atlinear concentration range 3.

0 – 50, and 10 – 30 μg/mL, with molar orptivity of 4.

377×103, 7.

446×103 L.

mol-1.

cm-1 using 2, 5-DMP and 4-TBP reagents respectively.

An acceptable accuracyrepresented by the relative standard deviation (RSD%) was achieved of a 0.

46 and 0.

37 for 2, 5-DMPand 4-TBP respectively.

The proposed method was successfully used for the determination ofceftriaxone sodium in pure and in pharmaceuticals.