Vitamin d receptors genes polymorphisms and oxidative DNA damage among Kurd patients with type 2 diabetes, Kurdistan region (Iraq)‎

Abstract EN

Background : Although the relationship between vitamin D deficiency and oxidative DNA damage has been previously reported, few studies have examined vitamin D receptor (VDR) gene polymorphisms for association with the risk of DNA damage.

This study aimed to identify vitamin D receptor genotypes in patients with type 2 diabetes and healthy subjects, as well as identify the relationship with oxidative DNA damage.

Methods: The study enrolled 162 subjects, 96 with type 2 diabetes and 66 healthy individuals were randomly selected to participate in prospective genotype detection by standard polymerase chain reaction methods and restriction fragment length polymorphism (PCR-RFLP).

The polymorphism of FokI and BsmI genes and its association with DNA damage were determined.

The main outcome measures were oxidative DNA damage marker including serum 8-hydroxy 2-deoxy guanosine (8-OHdG) and 25 hydroxy vitamin D [25(OH) D].

Results: Notable statistical significance exists in the frequency of genotype and allele of FokI (VDR 2228570 C>T) in patient group (OR 9.

7, P=0.

01) compared with the healthy individual group.

No significant difference was found in the patient group (OR 0.

74, p=0.

66) in the frequency of genotype and allele of BsmI (VDR 1544410 A>G.

).

The frequency allele carrier of the (VDR 2228570) C allele was higher in the patients sub- group with high DNA damage than in the healthy individuals (OR=1.

22, CI=0.

02-18.

9, p= 0.

70).

The frequency allele carrier of the (VDR 1544410) G allele was also higher in the patients sub-group with a high level of DNA damage than in the healthy individuals (OR=1.

38, CI=0.

13-16.

8, p=0.

57).

Conclusion: Our results suggest a significant relationship between DNA damage and the gene polymorphism FokI (VDR 228570 C>T) CC and its allele C among diabetic patients.

Additionally, our results suggest that the high prevalence of FokI (VDR 228570 C>T) polymorphism among patients group may be a genetic marker of susceptibility for diabetes in our population.