In vitro placenta stem cells isolation and in vivo differentiation on porous hydroxyapatite block in rabbits mandible

Abstract EN

This study intended to isolate placenta stem cell (PSCs) and, to assess its ability to differentiate into osteoblasts on the porous hydroxyapatite block (PHAB).

In addition to evaluating the bone formation around PHAB graft introduced in the mandible defect area using light (LM) and scanning electron microscopy (SEM).

A protocol was developed for isolation of PSCs, and osteogenic differentiation medium (ODM) was used for in vitro differentiation into osteoblasts.

Four New Zealand male rabbits were use to place the PSCs-PHA blocks (PSCs-PH) in the created defect on the body of the right mandible.

The left mandible was use as control where PHwithout cells (PHWCs) were introduced on the created defected area.

The implants retrieved at 60 days and processed for SEM and histological investigations.

No bone formation was seen in the control (PHWCs) implants.

Both LM and SEM revealed adherence of PSCs to PHin vitro after seven days of the incubation period.

Moreover, SEM verified the attachment and growth of the cells on the surface accompanied by invading in varying depths of PHmatrix.

Macroscopically, PSCs-PHretrieved implants revealed vascularization and bone-like tissue.

The mature bone development was seen in the histological slices, while, multiple layers of cells were seen in SEM accompanied by layers of collagen that covered the surface of PHIn conclusion, this study approved the isolation of PSCs and its differentiation into osteoblasts.

Moreover, PSCs can be considered as an easily reachable supply of multipotent stem cells for prospect trials and tissue engineering clinical purposes for the reconstruction of bone in the presence of appropriate scaffold material.