Detection of intracellular adhesion gene (icaa and icad)and biofilm formation staphylococcus aureus isolates from mastitis milk of sheep and goat
Other Title(s)
الكشف عن جینات الالتصاق الداخل خلویة و تكوین الغشاء الحیوي في جرثومة المكورات العنقودية المعزولة من حالات التھاب الضرع في الأغنام و الماعز
Parallel Title
الكشف عن جینات الالتصاق الداخل خلویة و تكوین الغشاء الحیوي في جرثومة المكورات العنقودية المعزولة من حالات التھاب الضرع في الأغنام و الماعز
Joint Authors
Adbis, Hasan Karim
Khidr, Muhammad Hasan
Source
Basrah Journal of Veterinary Research
Issue
Vol. 18, Issue 2 (31 Dec. 2019), pp.306-327, 22 p.
Publisher
University of Basrah College of Veterinary Medicine
Publication Date
2019-12-31
Country of Publication
Iraq
No. of Pages
22
Main Subjects
Topics
Abstract EN
In the present study, a total of 150 mastitis milk samples were collected from sheep andgoat (75 for each one) and were analyzed for the presence of S.
aureus.
.
The obtainedresults indicate that this bacterium observed in 20% of these samples (21.
33% from sheepand 18.
66% from goat).
The study of antibiotic susceptibility test to 9 different antibioticsshowed that S.
aureus was 100% resistant to penicillin and 100% sensitive tovancomycin, gentamycin, clarithromycin and chloramphenicol.
whereas for cefoxitin(alternative to methicillin) resistance was 47%.
There were a variable sensitivitypercentage for the rest of antibiotics: Tetracycline (70%), Ciprofloxacin (80%), Clindamycin (83%).
The biofilm-forming ability of S.
aureus was evaluated viamicrotiter plates and the result revealed that, all the studied isolates were either moderatebiofilm producer or weak biofilm producer while the non-biofilm producer and strongbiofilm producer were not detected among the tested isolate.
The relationship betweenbiofilm formation and resistance to methicillin showed there as no significant differences(P>0.
05) in the percentage of weak and moderate biofilm producers between MRSA andMSSA isolates.
PCR analysis was applied to DNA extracted from S.
aureus isolates frommilk samples.
The results of PCR assay revealed that all S.
aureus isolates gave positiveresults for both icaA and icaD genes (100%) with Product size 151 and 211 bp, respectively.
Results of this study indicate that biofilm producing S.
aureus have a major role player onthe occurrence of mastitis.
In addition, there was high prevalence of MRSA isolates(47%) in mastitic milk at the study area
American Psychological Association (APA)
Adbis, Hasan Karim& Khidr, Muhammad Hasan. 2019. Detection of intracellular adhesion gene (icaa and icad)and biofilm formation staphylococcus aureus isolates from mastitis milk of sheep and goat. Basrah Journal of Veterinary Research،Vol. 18, no. 2, pp.306-327.
https://search.emarefa.net/detail/BIM-973884
Modern Language Association (MLA)
Adbis, Hasan Karim& Khidr, Muhammad Hasan. Detection of intracellular adhesion gene (icaa and icad)and biofilm formation staphylococcus aureus isolates from mastitis milk of sheep and goat. Basrah Journal of Veterinary Research Vol. 18, no. 2 (2019), pp.306-327.
https://search.emarefa.net/detail/BIM-973884
American Medical Association (AMA)
Adbis, Hasan Karim& Khidr, Muhammad Hasan. Detection of intracellular adhesion gene (icaa and icad)and biofilm formation staphylococcus aureus isolates from mastitis milk of sheep and goat. Basrah Journal of Veterinary Research. 2019. Vol. 18, no. 2, pp.306-327.
https://search.emarefa.net/detail/BIM-973884
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 321-327
Record ID
BIM-973884