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New alternative method of drumstick tree (Moringa oleifera Lam.) micropropagation
Joint Authors
Mazru, Khalid al-Mursi
al-Atifi, Muhammad Said
Source
Journal of Productivity and Development
Issue
Vol. 18, Issue 3 (31 Aug. 2013), pp.339-348, 10 p.
Publisher
Zagazig University Faculty of Technology and Development
Publication Date
2013-08-31
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Tissue culture protocol reported in this study is an alternative method suggested to produce plantlets of drumstick tree (Moringa oleifera Lam.) with uniform genotypes for breeding, in vitro and in vivo production.
The highest rate of micropropagtion will often depend not only on the selection of the most suitable explants, but also on sterilization agent and the best basal medium for that tissue.
Sterilizing shoot tip explants of drumstick, using 15 % of clorex for 15 minutes showed the highest survival percentage and lowest contamination as well as sufficient browning percentage.
For multiple shoot induction, the shoot tip explants of drumstick were cultured on Murashige and Skoog (MS) agar medium supplemented with different concentrations of 2-isopentinyl adenine (2iP) at (0.0,0.5,1.0 and 2.0 mg/L) alone or in combination with indol butyric acid (IBA) at ( 0.0,0.1,0.5and 1.0 mg/ L).
The highest shoot multiplication rate was found in the 3rd subculture.
Moreover, the highest shoot number/explant (8.8) was obtained with the medium contained 0.5mg/l 2iP with 0.1mg/ L IBA and maintained at 25±2C compared to all other treatments.
The control treatment alone significantly showed the highest shoot length compared to all other treatments Low concentrations (0.5mg/L ) with (0.1ml/L IBA) were more effective in increasing the number of leaves.
Plantlets grown on the control medium produced the maximum number of healthy roots.
The rooted plantlets were hardened and successfully established in the soil
American Psychological Association (APA)
Mazru, Khalid al-Mursi& al-Atifi, Muhammad Said. 2013. New alternative method of drumstick tree (Moringa oleifera Lam.) micropropagation. Journal of Productivity and Development،Vol. 18, no. 3, pp.339-348.
https://search.emarefa.net/detail/BIM-974509
Modern Language Association (MLA)
Mazru, Khalid al-Mursi& al-Atifi, Muhammad Said. New alternative method of drumstick tree (Moringa oleifera Lam.) micropropagation. Journal of Productivity and Development Vol. 18, no. 3 (Aug. 2013), pp.339-348.
https://search.emarefa.net/detail/BIM-974509
American Medical Association (AMA)
Mazru, Khalid al-Mursi& al-Atifi, Muhammad Said. New alternative method of drumstick tree (Moringa oleifera Lam.) micropropagation. Journal of Productivity and Development. 2013. Vol. 18, no. 3, pp.339-348.
https://search.emarefa.net/detail/BIM-974509
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-974509