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In vitro antimicrobial activity and GC-MS analysis of crude aqueous methanolic extract produced from leaves of eucalyptus species
Other Title(s)
الفعالية المايكروبية و تحليل كروماتوغرافيا الغاز- الطيف الكتلي خارج جسم الكائن الحي لمستخلص الميثانول المائي الخام الذي تم الحصول عليه من اوراق نبات ال Eucalyptus species
Joint Authors
al-Hasan, Dirgham Ali Hasan
al-Badri , Majid Abd Zuwayd
al-Dafai, Husayn Ali Husayn
Source
Issue
Vol. 17, Issue 1 (30 Jun. 2019), pp.54-69, 16 p.
Publisher
University of Thi-Qar College of Medicine
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
16
Main Subjects
Topics
Abstract AR
تمثل النباتات احد المصادر المهمة للحصول على المنتجات ذات الاستخدام المفيد بما في ذلك المضادات المايكروبية.
إن الدراسة الحالية أظهرت فعالية ضد مايكروبية أبداها الميثانول المائي الخام بتركيز40000 مايكروغرام \ مل تم الحصول عليه من أوراق نبات الـ.
Eucalyptus sp ضد جراثيم الـ Staphylococcus aureus، Streptococcusmutans، Escherichiacoli و Klebsiellasp.
فضلا عن خميرة الـ Candida albiacns و بأقطار مناطق تثبيط سجلت بالأرقام 30، 27، 26، 30 و 38 ملم و بالترتيب.
الكشوفات الكيميائية اظهرت بان مستخلص الميثانول المائي الخام يحتوي على المواد Alkaloids، Glycosides، Flavonoids، Tannins و Saponins بالإضافة الى تحليل الـ GC-MS الذي كشف عن احتواء النباتي الخام على المركبات dimethylsulfoxonium ، formylmethylide, diethylphthalate, benzene, 1, 1'-(1,2-cyclobutanediyl)bis trans-,diisoctyladipate, and 2-methyl-7-phenylindole-.
إن الاستنتاج من هذه الدراسة توضح في وجود مركبات فعالة ضد الممرضات المجهرية ما يشير إلى وجوب فصلها كل على حده و بشكل نقي باستخدام تقنيات الكروماتوغرافيا لاختبار تلك المركبات النقية خارج و داخل الجسم و من ثم تشخيص تركيبها الكيميائي كي يكون بالإمكان استخدامها كأدوية لمعالجة الامراض في المستشفيات.
Abstract EN
Objectives : To evaluate the antimicrobial activity of the aqueous methanolic extract was given by the leaves of Eucalyptus sp.
growing in Thi-Qar Province, south of Iraq.
Additionally, to detect the compounds within the extract by using the technique of gas chromatography-mass spectrometry (GC-MS).
Methodology : The healthy leaves of Eucalyptus sp.
were collected, washed with tap water then by distilled water, dried, and pulverized through using a small mill to make the dried leaves as a powder.
The leaves powder was extracted by absolute petroleum ether then aqueous methanol (80 % ) for obtaining a sticky crude extract of the aqueous methanol filtrate.
The crude extract was tested against some microbial pathogens as well as analysis of the extract using the technique of GC-MS and phytochemical tests.
Results : The current study showed the antimicrobial activity of the crude aqueous methanolic extract obtained from Leaves of Eucalyptus sp.
by which 40000 µg ∕ ml of the extract exhibited inhibition zones around Staphylococcus aureus, Streptococcus mutans, Escherichia coli, and Klebsiella sp.
besides Candida albicans that the inhibition zones were measured to be 30, 27, 26, 30, and 38 mm respectively.
The chemical tests appeared the crude extract containsalkaloids, glycosides, flavonoids, tannins, andsaponin glycosides.
GC-MS analysis detected constituents of the extract are dimethylsulfoxoniumformylmethylide, diethyl phthalate, benzene, 1, 1'-(1, 2-cyclobutanediyl)bis-trans-, diisoctyladipate, and 2-methyl-7-phenylindole.
Conclusion : The present study concluded that the leaves ofEucalyptussp.
possess the bioactive products which are needed to be separatedas the pure compoundsby using techniques of chromatography in order to separately tested in vitro and in vivo, then application of spectrometry techniques for characterization of their chemical structure.
Finally, these compounds can be used to treat diseases as drugs in hospitals.
American Psychological Association (APA)
al-Dafai, Husayn Ali Husayn& al-Hasan, Dirgham Ali Hasan& al-Badri , Majid Abd Zuwayd. 2019. In vitro antimicrobial activity and GC-MS analysis of crude aqueous methanolic extract produced from leaves of eucalyptus species. Thi-Qar Medical Journal،Vol. 17, no. 1, pp.54-69.
https://search.emarefa.net/detail/BIM-976307
Modern Language Association (MLA)
al-Dafai, Husayn Ali Husayn…[et al.]. In vitro antimicrobial activity and GC-MS analysis of crude aqueous methanolic extract produced from leaves of eucalyptus species. Thi-Qar Medical Journal Vol. 17, no. 1 (2019), pp.54-69.
https://search.emarefa.net/detail/BIM-976307
American Medical Association (AMA)
al-Dafai, Husayn Ali Husayn& al-Hasan, Dirgham Ali Hasan& al-Badri , Majid Abd Zuwayd. In vitro antimicrobial activity and GC-MS analysis of crude aqueous methanolic extract produced from leaves of eucalyptus species. Thi-Qar Medical Journal. 2019. Vol. 17, no. 1, pp.54-69.
https://search.emarefa.net/detail/BIM-976307
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 65-68
Record ID
BIM-976307