Organogenesis of tomato (Lycopersicon Esculentum Mill. ) cv. sandra and rocky by hypocotyl, cotyledon and leaf explants.
Joint Authors
Daniyal, Gharbiyah Hurmuz
Ibrahim, Diya Ayyub
Source
Arab Journal of Agriculture Sciences
Issue
Vol. 3, Issue 5 (31 Jan. 2020), pp.31-53, 23 p.
Publisher
The Arab Institution for Education Science and Art
Publication Date
2020-01-31
Country of Publication
Egypt
No. of Pages
23
Main Subjects
Topics
Abstract EN
In vitro regeneration protocols were established for two Tomato (Lycopersicon esculentum Mill.) cultivars (Sandra and Rocky) grown in Kurdistan region using hypocotyl, cotyledonous leaves and leaf explants.
The explants excised from 12-15 days old of in vitro plantlets cultured on MS medium supplemented with different concentrations and combinations of plant growth regulators.
High organogenesis percentage (100 %) was achieved from hypocotyl grown in MS medium augmented with 1.0mgl-1 BA+ 0.5mgl-1 kinetin, which record approximately 3.75 in Sandra and 4.09 in Rocky cultivar as average number of shoots produced from each explant.
While the highest organogenesis percentage (100 %) was formed from cotyledon leaves using 1.5 mgl-1 BA + 0.5mgl-1 kinetin that gave around 3.5 in Sandra cultivar as average number of shoots / explant.
In contrast, the highest organogenesis was found on MS medium supplemented with 2.0mgl-1 BA + 1.0 mgl-1 kinetin and the average number of shoots produced from each explant were approximately 3.17 in Rocky cultivar.
The results of combination between 5.0mgl-1 BA with (0.0, 0.2, 0.5 and 1.0)mgl-1 NAA, indicated that the highest organogenesis percentage was recorded from hypocotyl (91.67 % with 6.5 shoots / explant and 66.67 % with 4.0 shoots / explant) in Sandra and Rocky cultivars respectively grown on MS medium supplemented with 5.0 mgl-1 BA alone.
While the highest organogenesis percentage (100 %) was induced from cotyledonous leaves in both cultivars (3.67 shoots for Sandra and 3.08 for Rocky) on MS medium supplemented with 5.0mgl-1 BA +0.2mgl-1 NAA.
Meanwhile, the highest organogenesis percentage was obtained from leaf explants for both cultivars on the combination of 1.0mgl-1 BA +0.5mgl-1 kinetin, (77.7 8% and 66.67 %) in Sandra and Rocky cultivars respectively.
The regenerated plants were successfully transplanted to the field for acclimatization.
American Psychological Association (APA)
Daniyal, Gharbiyah Hurmuz& Ibrahim, Diya Ayyub. 2020. Organogenesis of tomato (Lycopersicon Esculentum Mill. ) cv. sandra and rocky by hypocotyl, cotyledon and leaf explants.. Arab Journal of Agriculture Sciences،Vol. 3, no. 5, pp.31-53.
https://search.emarefa.net/detail/BIM-980492
Modern Language Association (MLA)
Daniyal, Gharbiyah Hurmuz& Ibrahim, Diya Ayyub. Organogenesis of tomato (Lycopersicon Esculentum Mill. ) cv. sandra and rocky by hypocotyl, cotyledon and leaf explants.. Arab Journal of Agriculture Sciences Vol. 3, no. 5 (Jan. 2020), pp.31-53.
https://search.emarefa.net/detail/BIM-980492
American Medical Association (AMA)
Daniyal, Gharbiyah Hurmuz& Ibrahim, Diya Ayyub. Organogenesis of tomato (Lycopersicon Esculentum Mill. ) cv. sandra and rocky by hypocotyl, cotyledon and leaf explants.. Arab Journal of Agriculture Sciences. 2020. Vol. 3, no. 5, pp.31-53.
https://search.emarefa.net/detail/BIM-980492
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 51-53
Record ID
BIM-980492