فعالية بعض محاليل التمديد في حفظ السائل المنوي لكباش العواس

Abstract EN

This study was carried out at the laboratory of artificial insemination and embryo transfer in Ezra station Belongs to ACSAD during 2009 to estimate the efficiency of local diluents (sodium citrate with egg yolk, EYC and skimmed milk, SM)in comparison to Andromed® as a standard diluent for short and long -term preservation period of semen in Awassi rams.

Four rams, two years old with live body weight 75± 3 Kg, were used.

Semen was collected by artificial insemination twice/ per day per week for 4 months during the breeding season.

Progressive motility and live – dead sperms were evaluated at each step of semen handling.

Results showed that there was a significant effect (p<0.05) for the ram on ejaculate volume (1.8-2.46 ml) and sperm concentration (2.4-3.8 X 109 /ml) and more significant effect (p< 0.01) for the diluents on studied traits after each step of semen handling.

The highest final survival rate of viability (65.1%) was shown in semen diluted with Andromed® compared with 51.3% in EYC and 22.3% in SM and the total rates of dead sperms were 41.9, 56.9.

78.9% in the standard, EYC and in SM, respectively.

It was concluded that local diluents can be used for extending and using Awassi semen as a fresh diluted semen for a short time preservation using SM and as a frozen and preserved semen for a long time with EYC in artificial insemination programs and it was suggested that EYC with glycerol can be replaced for Andromed® if sperm concentrate is increased by 10-15% at dilution.