Distinct Effects of Contraction Agonists on the Phosphorylation State of Cofilin in Pulmonary Artery Smooth Muscle

Abstract EN

We hypothesized that agonist-induced contraction correlates with the phospho-cofilin/cofilin (P-CF/CF) ratio in pulmonary artery (PA) rings and cultured smooth muscle cells (PASMCs).

PA rings were used for isometric contractions and along with PASMCs for assay of P-CF/CF by isoelectric focusing and immunoblotting.

The P-CF/CF measured 22.5% in PA and differentiated PASMCs, but only 14.8% in undifferentiated PASMCs.

With comparable contraction responses in PA, endothelin-1 (100 nM) and norepinephrine (1 μM) induced a 2-fold increase of P-CF/CF, while angiotensin II (1 μM) induced none.

All agonists activated Rho-kinase and LIMK2, and activation was eliminated by inhibition of Rho-kinase.

Microcystin LF (20 nM) potentiated the angiotensin II, but not the 5-hydroxytryptamine (1 μM)-mediated increase of P-CF/CF.

In conclusion, all tested agonists activate the Rho-kinase-LIMK pathway and increase P-CF/CF.

Angiotensin II activates PP2A and counteracts the LIMK-mediated CF phosphorylation.

CF phosphorylation stabilizes peripheral actin structures and may contribute to the maximal contraction of PA.