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Cu(II) and Ni(II) Interactions with the Terminally Blocked Hexapeptide Ac-Leu-Ala-His-Tyr-Asn-Lys-amide Model of Histone H2B (80–85)
Joint Authors
Panagiotou, Katerina
Hagarman, Andrew
Schweitzer-Stenner, Reinhard
Hadjiliadis, Nick
Malandrinos, Gerasimos
Dokorou, Vassiliki
Karavelas, Tilemachos
Panagopoulou, Maria
Soffer, Jonathan
Source
Bioinorganic Chemistry and Applications
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-02-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The N- and C-terminal blocked hexapeptide Ac-Leu-Ala-His-Tyr-Asn-Lys-amide (LAHYNK) representing the 80–85 fragment of histone H2B was synthesized and its interactions with Cu(II) and Ni(II) ions were studied by potentiometric, UV-Vis, CD, EPR, and NMR spectroscopic techniques in solution.
Our data reveal that the imidazole N(3) nitrogen atom is the primary ligating group for both metal ions.
Sequential amide groups deprotonation and subsequent coordination to metal ions indicated an {Nimidazole,3Namide} coordination mode above pH∼9, in all cases.
In the case of Cu(II)-peptide system, the almost exclusive formation of the predominant species CuL in neutral media accounting for almost 98% of the total metal ion concentration at pH 7.3 strongly indicates that at physiological pH values the sequence -LAHYNK- of histone H2B provides very efficient binding sites for metal ions.
The imidazole pyrrole N(1) ionization (but not coordination) was also detected in species CuH-4L present in solution above pH ∼ 11.
American Psychological Association (APA)
Panagiotou, Katerina& Panagopoulou, Maria& Karavelas, Tilemachos& Dokorou, Vassiliki& Hagarman, Andrew& Soffer, Jonathan…[et al.]. 2008. Cu(II) and Ni(II) Interactions with the Terminally Blocked Hexapeptide Ac-Leu-Ala-His-Tyr-Asn-Lys-amide Model of Histone H2B (80–85). Bioinorganic Chemistry and Applications،Vol. 2008, no. 2008, pp.1-10.
https://search.emarefa.net/detail/BIM-987746
Modern Language Association (MLA)
Panagiotou, Katerina…[et al.]. Cu(II) and Ni(II) Interactions with the Terminally Blocked Hexapeptide Ac-Leu-Ala-His-Tyr-Asn-Lys-amide Model of Histone H2B (80–85). Bioinorganic Chemistry and Applications No. 2008 (2008), pp.1-10.
https://search.emarefa.net/detail/BIM-987746
American Medical Association (AMA)
Panagiotou, Katerina& Panagopoulou, Maria& Karavelas, Tilemachos& Dokorou, Vassiliki& Hagarman, Andrew& Soffer, Jonathan…[et al.]. Cu(II) and Ni(II) Interactions with the Terminally Blocked Hexapeptide Ac-Leu-Ala-His-Tyr-Asn-Lys-amide Model of Histone H2B (80–85). Bioinorganic Chemistry and Applications. 2008. Vol. 2008, no. 2008, pp.1-10.
https://search.emarefa.net/detail/BIM-987746
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-987746