Preparation and Characterization of a Novel Chimeric Protein VEGI-CTT in Escherichia coli
Joint Authors
Wei, Ruili
Cheng, Jinwei
Cai, Jiping
Source
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-07-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Vascular endothelial cell growth inhibitor (VEGI) is a recently identified antiangiogenic cytokine that belongs to the TNF superfamily, and could effectively inhibit endothelial cell proliferation and angiogenesis.
Synthetic peptide CTT (CTTHWGFTLC) has been found to suppress invasion and migration of both tumor and endothelial cells by potent and selective inhibition of MMP-2 and MMP-9.
To prepare chimeric protein VEGI-CTT for more potent antitumor therapy, the recombinant expression vector pET-VEGI-CTT was constructed.
This fusion protein was expressed in inclusion bodies in E.
coli BL21 (DE3), and was refolded and purified by immobilized metal affinity chromatography using His-tag.
Purified VEGI-CTT protein was characterized by proliferation assays of the endothelial cells and casein degradation assay in vitro.
The results demonstrated that chimeric protein VEGI-CTT had a potent activity of antiangiogenesis through inhibiting the proliferation of endothelial cells, and could effectively reduce the activity of MMP-2 and MMP-9.
The preliminarily in vivo study demonstrated that chimeric protein VEGI-CTT had more potent antitumor activity than VEGI and/or CTT peptide against CA46 human lymphoma xenografts in nude mice.
Thus, these facts that are derived from the present study suggest that the chimeric protein VEGI-CTT may be used for tumor therapy in the future.
American Psychological Association (APA)
Cai, Jiping& Wei, Ruili& Cheng, Jinwei. 2008. Preparation and Characterization of a Novel Chimeric Protein VEGI-CTT in Escherichia coli. BioMed Research International،Vol. 2008, no. 2008, pp.1-9.
https://search.emarefa.net/detail/BIM-987789
Modern Language Association (MLA)
Cai, Jiping…[et al.]. Preparation and Characterization of a Novel Chimeric Protein VEGI-CTT in Escherichia coli. BioMed Research International No. 2008 (2008), pp.1-9.
https://search.emarefa.net/detail/BIM-987789
American Medical Association (AMA)
Cai, Jiping& Wei, Ruili& Cheng, Jinwei. Preparation and Characterization of a Novel Chimeric Protein VEGI-CTT in Escherichia coli. BioMed Research International. 2008. Vol. 2008, no. 2008, pp.1-9.
https://search.emarefa.net/detail/BIM-987789
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-987789