![](/images/graphics-bg.png)
The Cyclodextrin Glycosyltransferase of Paenibacillus pabuli US132 Strain: Molecular Characterization and Overproduction of the Recombinant Enzyme
Joint Authors
Bejar, Samir
Jemli, Sonia
Ben Mabrouk, Sameh
Ben Messaoud, Ezzedine
Source
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-07-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The gene encoding the cyclodextrin glycosyltransferase (CGTase) of Paenibacillus pabuli US132, previously described as efficient antistaling agent and good candidate for cyclodextrins production, was cloned, sequenced, and expressed in Escherichia coli.
Sequence analysis showed that the mature enzyme (684 amino acids) was preceded by a signal peptide of 34 residues.
The enzyme exhibited the highest identity (94%) to the β-CGTase of Bacillus circulans no.
8.
The production of the recombinant CGTase, as active form, was very low (about 1 U/mL) in shake flasks at 37∘C.
This production reached 22 U/mL after 22 hours of induction by mainly shifting the postinduction temperature from 37 to 19∘C and using 2TY instead of LB medium.
High enzyme production (35 U/mL) was attained after 18 hours of induction in fermentor using the same culture conditions as in shake flask.
The recombinant enzyme showed Vmax and Km values of 253±36 μmol of β-cyclodextrin/mg/min and 0.36±0.18 g/L, respectively.
American Psychological Association (APA)
Jemli, Sonia& Ben Messaoud, Ezzedine& Ben Mabrouk, Sameh& Bejar, Samir. 2008. The Cyclodextrin Glycosyltransferase of Paenibacillus pabuli US132 Strain: Molecular Characterization and Overproduction of the Recombinant Enzyme. BioMed Research International،Vol. 2008, no. 2008, pp.1-9.
https://search.emarefa.net/detail/BIM-987796
Modern Language Association (MLA)
Jemli, Sonia…[et al.]. The Cyclodextrin Glycosyltransferase of Paenibacillus pabuli US132 Strain: Molecular Characterization and Overproduction of the Recombinant Enzyme. BioMed Research International No. 2008 (2008), pp.1-9.
https://search.emarefa.net/detail/BIM-987796
American Medical Association (AMA)
Jemli, Sonia& Ben Messaoud, Ezzedine& Ben Mabrouk, Sameh& Bejar, Samir. The Cyclodextrin Glycosyltransferase of Paenibacillus pabuli US132 Strain: Molecular Characterization and Overproduction of the Recombinant Enzyme. BioMed Research International. 2008. Vol. 2008, no. 2008, pp.1-9.
https://search.emarefa.net/detail/BIM-987796
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-987796