Proteome-Level Responses of Escherichia coli to Long-Chain Fatty Acids and Use of Fatty Acid Inducible Promoter in Protein Production
Joint Authors
Han, Mee-Jung
Lee, Jeong Wook
Lee, Sang Yup
Yoo, Jong Shin
Source
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2007-11-21
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
In Escherichia coli, a long-chain acyl-CoA is a regulatory signal that modulates gene expression through its binding to a transcription factor FadR.
In this study, comparative proteomic analysis of E.
coli in the presence of glucose and oleic acid was performed to understand cell physiology in response to oleic acid.
Among total of 52 proteins showing altered expression levels with oleic acid presence, 9 proteins including AldA, Cdd, FadA, FadB, FadL, MalE, RbsB, Udp, and YccU were newly synthesized.
Among the genes that were induced by oleic acid, the promoter of the aldA gene was used for the production of a green fluorescent protein (GFP).
Analysis of fluorescence intensities and confocal microscopic images revealed that soluble GFP was highly expressed under the control of the aldA promoter.
These results suggest that proteomics is playing an important role not only in biological research but also in various biotechnological applications.
American Psychological Association (APA)
Han, Mee-Jung& Lee, Jeong Wook& Lee, Sang Yup& Yoo, Jong Shin. 2007. Proteome-Level Responses of Escherichia coli to Long-Chain Fatty Acids and Use of Fatty Acid Inducible Promoter in Protein Production. BioMed Research International،Vol. 2008, no. 2008, pp.1-13.
https://search.emarefa.net/detail/BIM-987799
Modern Language Association (MLA)
Han, Mee-Jung…[et al.]. Proteome-Level Responses of Escherichia coli to Long-Chain Fatty Acids and Use of Fatty Acid Inducible Promoter in Protein Production. BioMed Research International No. 2008 (2008), pp.1-13.
https://search.emarefa.net/detail/BIM-987799
American Medical Association (AMA)
Han, Mee-Jung& Lee, Jeong Wook& Lee, Sang Yup& Yoo, Jong Shin. Proteome-Level Responses of Escherichia coli to Long-Chain Fatty Acids and Use of Fatty Acid Inducible Promoter in Protein Production. BioMed Research International. 2007. Vol. 2008, no. 2008, pp.1-13.
https://search.emarefa.net/detail/BIM-987799
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-987799