Subcellular Localization and RNA Interference of an RNA Methyltransferase Gene from Silkworm, Bombyx Mori
Joint Authors
Zhang, Yaozhou
Lv, Zhengbing
He, Pingan
Chen, Jian
Wang, Dan
Wu, Xiangfu
Nie, Zuoming
Zhou, Ruobing
Shen, Hongdan
Wang, Xuedong
Source
International Journal of Genomics
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-04-07
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
RNA methylation, which is a form of posttranscriptional modification, is catalyzed by S-adenosyl-L-methionone-dependent RNA methyltransterases (RNA MTases).
We have identified a novel silkworm gene, BmRNAMTase, containing a 369-bp open reading frame that encodes a putative protein containing 122 amino acid residues and having a molecular weight of 13.88 kd.
We expressed a recombinant His-tagged BmRNAMTase in E.
coli BL21 (DE3), purified the fusion protein by metal-chelation affinity chromatography, and injected a New Zealand rabbit with the purified protein to generate anti-BmRNAMTase polyclonal antibodies.
Immunohistochemistry revealed that BmRNAMTase is abundant in the cytoplasm of Bm5 cells.
In addition, using RNA interference to reduce the intracellular activity and content of BmRNAMTase, we determined that this cytoplasmic RNA methyltransferase may be involved in preventing cell death in the silkworm.
American Psychological Association (APA)
Nie, Zuoming& Zhou, Ruobing& Chen, Jian& Wang, Dan& Lv, Zhengbing& He, Pingan…[et al.]. 2008. Subcellular Localization and RNA Interference of an RNA Methyltransferase Gene from Silkworm, Bombyx Mori. International Journal of Genomics،Vol. 2008, no. 2008, pp.1-7.
https://search.emarefa.net/detail/BIM-987872
Modern Language Association (MLA)
Nie, Zuoming…[et al.]. Subcellular Localization and RNA Interference of an RNA Methyltransferase Gene from Silkworm, Bombyx Mori. International Journal of Genomics No. 2008 (2008), pp.1-7.
https://search.emarefa.net/detail/BIM-987872
American Medical Association (AMA)
Nie, Zuoming& Zhou, Ruobing& Chen, Jian& Wang, Dan& Lv, Zhengbing& He, Pingan…[et al.]. Subcellular Localization and RNA Interference of an RNA Methyltransferase Gene from Silkworm, Bombyx Mori. International Journal of Genomics. 2008. Vol. 2008, no. 2008, pp.1-7.
https://search.emarefa.net/detail/BIM-987872
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-987872