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Human Islet Amyloid Polypeptide Transgenic Mice: In Vivo and Ex Vivo Models for the Role of hIAPP in Type 2 Diabetes Mellitus
Joint Authors
Wierup, N.
Jacobs, H. M.
Sotthewes, G.
Berger, R.
Ahrén, Bo
Höppener, Jo W. M.
de Vos, P.
Sprong, M.
Sundler, Frank
Source
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-03-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Human islet amyloid polypeptide (hIAPP), a pancreatic islet protein of 37 amino acids, is the main component of islet amyloid, seen at autopsy in patients with type 2 diabetes mellitus (DM2).
To investigate the roles of hIAPP and islet amyloid in DM2, we generated transgenic mice expressing hIAPP in their islet beta cells.
In this study, we found that after a long-term, high-fat diet challenge islet amyloid was observed in only 4 of 19 hIAPP transgenic mice.
hIAPP transgenic females exhibited severe glucose intolerance, which was associated with a downregulation of GLUT-2 mRNA expression.
In isolated islets from hIAPP males cultured for 3 weeks on high-glucose medium, the percentage of amyloid containing islets increased from 5.5% to 70%.
This ex vivo system will allow a more rapid, convenient, and specific study of factors influencing islet amyloidosis as well as of therapeutic strategies to interfere with this pathological process.
American Psychological Association (APA)
Höppener, Jo W. M.& Jacobs, H. M.& Wierup, N.& Sotthewes, G.& Sprong, M.& de Vos, P.…[et al.]. 2008. Human Islet Amyloid Polypeptide Transgenic Mice: In Vivo and Ex Vivo Models for the Role of hIAPP in Type 2 Diabetes Mellitus. Journal of Diabetes Research،Vol. 2008, no. 2008, pp.1-8.
https://search.emarefa.net/detail/BIM-988017
Modern Language Association (MLA)
Höppener, Jo W. M.…[et al.]. Human Islet Amyloid Polypeptide Transgenic Mice: In Vivo and Ex Vivo Models for the Role of hIAPP in Type 2 Diabetes Mellitus. Journal of Diabetes Research No. 2008 (2008), pp.1-8.
https://search.emarefa.net/detail/BIM-988017
American Medical Association (AMA)
Höppener, Jo W. M.& Jacobs, H. M.& Wierup, N.& Sotthewes, G.& Sprong, M.& de Vos, P.…[et al.]. Human Islet Amyloid Polypeptide Transgenic Mice: In Vivo and Ex Vivo Models for the Role of hIAPP in Type 2 Diabetes Mellitus. Journal of Diabetes Research. 2008. Vol. 2008, no. 2008, pp.1-8.
https://search.emarefa.net/detail/BIM-988017
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988017