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Improved Methodology for Monitoring Poly(amidoamine) Dendrimers Surface Transformations and Product Quality by Ultra Performance Liquid Chromatography
Joint Authors
Haik, Kristi L.
Girten, Craig D.
Bullen, Heather A.
Walters, Keith A.
Tomalia, Donald A.
Fabré, Thomas A.
Oehrle, Stuart A.
Cason, Chevelle A.
Source
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-03-16
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Ultra performance liquid chromatography (UPLC) analysis was utilized for the first time as a methodology for monitoring poly(amidoamine) (PAMAM) dendrimer surface transformations and product quality.
Results were compared to high-performance liquid chromatography (HPLC) and were found to provide a vastly improved analytical method for the characterization of dendrimer polydispersity and variance in a typical surface modification.
The application of UPLC increased the average number of theoretical plates by a factor of 7 and reduced retention times of analytes by 36%, while improving the resolution capability to discriminate surface variances in dendrimers.
The new UPLC procedures were used to monitor surface modification of [core: ethylenediamine]; (G = 4); dendri-poly(amidoamine)-(NH2)64 (i.e., [EDA]; (G4); dendri-PAMAM-(NH2)64) to produce biotinylated dendrimer conjugates.
The enhanced sensitivity and efficiency of the UPLC analyses allowed resolution of biotin substituent levels and a better characterization of the targeted dendrimer conjugates compared to traditional HPLC methodology.
American Psychological Association (APA)
Cason, Chevelle A.& Oehrle, Stuart A.& Fabré, Thomas A.& Girten, Craig D.& Walters, Keith A.& Tomalia, Donald A.…[et al.]. 2008. Improved Methodology for Monitoring Poly(amidoamine) Dendrimers Surface Transformations and Product Quality by Ultra Performance Liquid Chromatography. Journal of Nanomaterials،Vol. 2008, no. 2008, pp.1-7.
https://search.emarefa.net/detail/BIM-988098
Modern Language Association (MLA)
Cason, Chevelle A.…[et al.]. Improved Methodology for Monitoring Poly(amidoamine) Dendrimers Surface Transformations and Product Quality by Ultra Performance Liquid Chromatography. Journal of Nanomaterials No. 2008 (2008), pp.1-7.
https://search.emarefa.net/detail/BIM-988098
American Medical Association (AMA)
Cason, Chevelle A.& Oehrle, Stuart A.& Fabré, Thomas A.& Girten, Craig D.& Walters, Keith A.& Tomalia, Donald A.…[et al.]. Improved Methodology for Monitoring Poly(amidoamine) Dendrimers Surface Transformations and Product Quality by Ultra Performance Liquid Chromatography. Journal of Nanomaterials. 2008. Vol. 2008, no. 2008, pp.1-7.
https://search.emarefa.net/detail/BIM-988098
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988098