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A New FRET-Based Sensitive DNA Sensor for Medical Diagnostics using PNA Probe and Water-Soluble Blue Light Emitting Polymer
Joint Authors
Aneja, Anamika
Mathur, Nidhi
Mathur, P. C.
Bhatnagar, P. K.
Source
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-07-08
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A reliable, fast, and low-cost biosensor for medical diagnostics using DNA sequence detection has been developed and tested for the detection of the bacterium “Bacillus anthracis.” In this sensor, Poly [9,9-di (6,6′- N, N′ trimethylammonium) hexylfluorenyl-2, 7-diyl)-alt-co- (1,4-phenylene)] dibromide salt (PFP) has been taken as cationic conjugated polymer (CCP) and PNA attached with fluorescein dye (PNAC∗) as a probe.
The basic principle of this sensor is that when a PNAC∗ probe is hybridized with a single strand DNA (ssDNA) having complementary sequence, Forster resonance energy transfer (FRET) may take place from PFP to the PNAC∗/DNA complex.
If the FRET is efficient, the photoluminescence from the PFP will be highly quenched and that from PNAC∗ will be enhanced.
On the other hand, if the DNA sequence is noncomplementary to PNA, FRET will not occur.
American Psychological Association (APA)
Mathur, Nidhi& Aneja, Anamika& Bhatnagar, P. K.& Mathur, P. C.. 2008. A New FRET-Based Sensitive DNA Sensor for Medical Diagnostics using PNA Probe and Water-Soluble Blue Light Emitting Polymer. Journal of Sensors،Vol. 2008, no. 2008, pp.1-6.
https://search.emarefa.net/detail/BIM-988134
Modern Language Association (MLA)
Mathur, Nidhi…[et al.]. A New FRET-Based Sensitive DNA Sensor for Medical Diagnostics using PNA Probe and Water-Soluble Blue Light Emitting Polymer. Journal of Sensors No. 2008 (2008), pp.1-6.
https://search.emarefa.net/detail/BIM-988134
American Medical Association (AMA)
Mathur, Nidhi& Aneja, Anamika& Bhatnagar, P. K.& Mathur, P. C.. A New FRET-Based Sensitive DNA Sensor for Medical Diagnostics using PNA Probe and Water-Soluble Blue Light Emitting Polymer. Journal of Sensors. 2008. Vol. 2008, no. 2008, pp.1-6.
https://search.emarefa.net/detail/BIM-988134
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988134