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Mycoplasma genitalium Lipoproteins Induce Human Monocytic Cell Expression of Proinflammatory Cytokines and Apoptosis by Activating Nuclear Factor κB
Joint Authors
Deng, Zhongliang
You, Xiaoxing
Zhu, Cuiming
Yu, Minjun
Qiu, Hong
Zeng, Yanhua
Wu, Yimou
Source
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-03-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
This study was designed to investigate the molecular mechanisms responsible for the induction of proinflammatory cytokines gene expression and apoptosis in human monocytic cell line THP-1 stimulated by lipoproteins (LPs) prepared from Mycoplasma genitalium.
Cultured cells were stimulated with M.
genitalium LP to analyze the production of proinflammatory cytokines and expression of their mRNA by ELISA and RT-PCR, respectively.
Cell apoptosis was also detected by Annexin V-FITC-propidium iodide (PI) staining and acridine orange (AO)-ethidium bromide (EB) staining.
The DNA-binding activity of nuclear factor-κB (NF-κB) was assessed by electrophoretic mobility shift assay (EMSA).
Results showed that LP stimulated THP-1 cells to produce tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6 in a dose-dependent manner.
The mRNA levels were also upregulated in response to LP stimulation.
LPs were also found to increase the DNA-binding activity of NF-κB, a possible mechanism for the induction of cytokine mRNA expression and the cell apoptosis.
These effects were abrogated by PDTC, an inhibitor of NF-κB.
Our results indicate that M.
genitalium-derived LP may be an important etiological factor of certain diseases due to the ability of LP to produce proinflammatory cytokines and induction of apoptosis, which is probably mediated through the activation of NF-κB.
American Psychological Association (APA)
Wu, Yimou& Qiu, Hong& Zeng, Yanhua& You, Xiaoxing& Deng, Zhongliang& Yu, Minjun…[et al.]. 2008. Mycoplasma genitalium Lipoproteins Induce Human Monocytic Cell Expression of Proinflammatory Cytokines and Apoptosis by Activating Nuclear Factor κB. Mediators of Inflammation،Vol. 2008, no. 2008, pp.1-8.
https://search.emarefa.net/detail/BIM-988138
Modern Language Association (MLA)
Wu, Yimou…[et al.]. Mycoplasma genitalium Lipoproteins Induce Human Monocytic Cell Expression of Proinflammatory Cytokines and Apoptosis by Activating Nuclear Factor κB. Mediators of Inflammation No. 2008 (2008), pp.1-8.
https://search.emarefa.net/detail/BIM-988138
American Medical Association (AMA)
Wu, Yimou& Qiu, Hong& Zeng, Yanhua& You, Xiaoxing& Deng, Zhongliang& Yu, Minjun…[et al.]. Mycoplasma genitalium Lipoproteins Induce Human Monocytic Cell Expression of Proinflammatory Cytokines and Apoptosis by Activating Nuclear Factor κB. Mediators of Inflammation. 2008. Vol. 2008, no. 2008, pp.1-8.
https://search.emarefa.net/detail/BIM-988138
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988138