Construction of a Recombinant Eukaryotic Expression Plasmid Containing Human Calcitonin Gene and Its Expression in NIH3T3 Cells
Joint Authors
Wu, Dan
Jiang, Guozhong
Li, Xiaolin
Wang, Xiuli
Zeng, Bingfang
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-08-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Aim.
To construct a recombinant eukaryotic expression plasmid containing human calcitonin (hCT) gene and express the gene in murine fibroblast NIH3T3 cells.
Materials and Methods.
A murine Igκ-chain leader sequence and hCT gene were synthesized and cloned into pCDNA3.0 to form the pCDNA3.0-Igκ-hCT eukaryotic expression vector, which was transfected into NIH3T3 cells.
The mRNA and protein expressions and secretion of hCT were detected.
Primarily cultured osteoclasts were incubated with the supernatant of pCDNA3.0-Igk-hCT-transfected NIH3T3 cells, and their numbers were counted and morphology observed.
Results.
The expression and secretion of hCT were successfully detected in pCDNA3.0-Igk-hCT-transfected NIH3T3 cells.
The number of osteoclasts was decreased and the cells became crumpled when they were incubated with the supernatant of pCDNA3.0-Igk-hCT-transfected NIH3T3 cells.
Conclusion.
A recombinant eukaryotic expression vector containing hCT gene was successfully constructed and expressed in NIH3T3 cells.
The secreted recombinant hCT inhibited the growth and morphology of osteoclasts.
American Psychological Association (APA)
Li, Xiaolin& Jiang, Guozhong& Wu, Dan& Wang, Xiuli& Zeng, Bingfang. 2009. Construction of a Recombinant Eukaryotic Expression Plasmid Containing Human Calcitonin Gene and Its Expression in NIH3T3 Cells. BioMed Research International،Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-988342
Modern Language Association (MLA)
Li, Xiaolin…[et al.]. Construction of a Recombinant Eukaryotic Expression Plasmid Containing Human Calcitonin Gene and Its Expression in NIH3T3 Cells. BioMed Research International No. 2009 (2009), pp.1-7.
https://search.emarefa.net/detail/BIM-988342
American Medical Association (AMA)
Li, Xiaolin& Jiang, Guozhong& Wu, Dan& Wang, Xiuli& Zeng, Bingfang. Construction of a Recombinant Eukaryotic Expression Plasmid Containing Human Calcitonin Gene and Its Expression in NIH3T3 Cells. BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-988342
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988342