Efficient siRNA Delivery by the Cationic Liposome DOTAP in Human Hematopoietic Stem Cells Differentiating into Dendritic Cells
Joint Authors
Martino, Sabata
Orlacchio, Aldo
D'Angelo, Francesco
di Girolamo, Ilaria
Tiribuzi, Roberto
Datti, Alessandro
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-05-31
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
RNA interference technology is an ideal strategy to elucidate the mechanisms associated with human CD34+ hematopoietic stem cell differentiation into dendritic cells.
Simple manipulations in vitro can unequivocally yield alloreactive or tolerogenic populations, suggesting key implications of biochemical players that might emerge as therapeutic targets for cancer or graft-versus-host disease.
To knockdown proteins typically involved in the biology of dendritic cells, we employed an siRNA delivery system based on the cationic liposome DOTAP as the carrier.
Freshly-isolated CD34+ cells were transfected with siRNA for cathepsin S with negligible cytotoxicity and transfection rates (>60%) comparable to the efficiency shown by lentiviral vectors.
Further, cathepsin S knockdown was performed during both cell commitment and through the entire 14-day differentiation process with repeated transfection rounds that had no effect per se on cell development.
Tested in parallel, other commercially-available chemical reagents failed to meet acceptable standards.
In addition to safe and practical handling, a direct advantage of DOTAP over viral-mediated techniques is that transient silencing effects can be dynamically appraised through the recovery of targeted proteins.
Thus, our findings identify DOTAP as an excellent reagent for gene silencing in resting and differentiating CD34+ cells, suggesting a potential for applications in related preclinical models.
American Psychological Association (APA)
Martino, Sabata& di Girolamo, Ilaria& Tiribuzi, Roberto& D'Angelo, Francesco& Datti, Alessandro& Orlacchio, Aldo. 2009. Efficient siRNA Delivery by the Cationic Liposome DOTAP in Human Hematopoietic Stem Cells Differentiating into Dendritic Cells. BioMed Research International،Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-988365
Modern Language Association (MLA)
Martino, Sabata…[et al.]. Efficient siRNA Delivery by the Cationic Liposome DOTAP in Human Hematopoietic Stem Cells Differentiating into Dendritic Cells. BioMed Research International No. 2009 (2009), pp.1-7.
https://search.emarefa.net/detail/BIM-988365
American Medical Association (AMA)
Martino, Sabata& di Girolamo, Ilaria& Tiribuzi, Roberto& D'Angelo, Francesco& Datti, Alessandro& Orlacchio, Aldo. Efficient siRNA Delivery by the Cationic Liposome DOTAP in Human Hematopoietic Stem Cells Differentiating into Dendritic Cells. BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-988365
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988365