Oxaprozin-Induced Apoptosis on CD40 Ligand-Treated Human Primary Monocytes Is Associated with the Modulation of Defined Intracellular Pathways
Joint Authors
Montecucco, Fabrizio
Bertolotto, Maria
Dallegri, Franco
Mach, François
Quercioli, Alessandra
Ottonello, Luciano
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-08-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The modulation of CD40L activity might represent a promising therapeutic target to reduce monocyte inflammatory functions in chronic diseases, such as rheumatoid arthritis.
In the present study, we investigated the possible influence of nonsteroidal anti-inflammatory drugs (NSAIDs) on CD40L-induced monocyte survival.
Monocytes were isolated from buffy coats by using Ficoll-Percoll gradients.
Monocyte apoptosis was evaluated by fluorescence microscopy on cytopreps stained with acridine orange or using flow cytometry analysis of Annexin-V and Propidium Iodide staining.
Akt and NF-κB activation was assessed using western blot.
Caspase 3 activity was determined spectrophotometrically.
Among different NSAIDs, only oxaprozin dose-dependently increased apoptosis of CD40L-treated monocytes.
Oxaprozin pro-apoptotic activity was associated with the inhibition of CD40L-triggered Akt and NF-κB phosphorylation and the activation of caspase 3.
In conclusion, our data suggest that oxaprozin-induced apoptosis in CD40L-treated human monocytes is associated with previously unknown cyclooxygenase (COX)-independent pathways.
These intracellular proteins might be promising pharmacological targets to increase apoptosis in CD40L-treated monocytes.
American Psychological Association (APA)
Montecucco, Fabrizio& Bertolotto, Maria& Ottonello, Luciano& Quercioli, Alessandra& Mach, François& Dallegri, Franco. 2009. Oxaprozin-Induced Apoptosis on CD40 Ligand-Treated Human Primary Monocytes Is Associated with the Modulation of Defined Intracellular Pathways. BioMed Research International،Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-988379
Modern Language Association (MLA)
Montecucco, Fabrizio…[et al.]. Oxaprozin-Induced Apoptosis on CD40 Ligand-Treated Human Primary Monocytes Is Associated with the Modulation of Defined Intracellular Pathways. BioMed Research International No. 2009 (2009), pp.1-9.
https://search.emarefa.net/detail/BIM-988379
American Medical Association (AMA)
Montecucco, Fabrizio& Bertolotto, Maria& Ottonello, Luciano& Quercioli, Alessandra& Mach, François& Dallegri, Franco. Oxaprozin-Induced Apoptosis on CD40 Ligand-Treated Human Primary Monocytes Is Associated with the Modulation of Defined Intracellular Pathways. BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-988379
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988379