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A Significant Increase of RNAi Efficiency in Human Cells by the CMV Enhancer with a tRNAlys Promoter
Joint Authors
Hang, Ning
Feng, Liu
Weiwei, Ma
Zhenhua, Xie
Yuyang, Jiang
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-09-16
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
RNA interference (RNAi) is the process of mRNA degradation induced by double-stranded RNA in a sequence-specific manner.
Different types of promoters, such as U6, H1, tRNA, and CMV, have been used to control the inhibitory effect of RNAi expression vectors.
In the present study, we constructed two shRNA expression vectors, respectively, controlled by tRNAlys and CMV enhancer-tRNAlys promoters.
Compared to the vectors with tRNAlys or U6 promoter, the vector with a CMV enhancer-tRNAlys promoter silenced pokemon more efficiently on both the mRNA and the protein levels.
Meanwhile, the silencing of pokemon inhibited the proliferation of MCF7 cells, but the induction of apoptosis of MCF7 cells was not observed.
We conclude that the CMV enhancer-tRNAlys promoter may be a powerful tool in driving intracellular expression of shRNA which can efficiently silence targeted gene.
American Psychological Association (APA)
Weiwei, Ma& Zhenhua, Xie& Feng, Liu& Hang, Ning& Yuyang, Jiang. 2009. A Significant Increase of RNAi Efficiency in Human Cells by the CMV Enhancer with a tRNAlys Promoter. BioMed Research International،Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-988384
Modern Language Association (MLA)
Weiwei, Ma…[et al.]. A Significant Increase of RNAi Efficiency in Human Cells by the CMV Enhancer with a tRNAlys Promoter. BioMed Research International No. 2009 (2009), pp.1-7.
https://search.emarefa.net/detail/BIM-988384
American Medical Association (AMA)
Weiwei, Ma& Zhenhua, Xie& Feng, Liu& Hang, Ning& Yuyang, Jiang. A Significant Increase of RNAi Efficiency in Human Cells by the CMV Enhancer with a tRNAlys Promoter. BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-988384
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988384