Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus
Joint Authors
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-08-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Chemokine receptor 4 (CXCR4) belongs to the large superfamily of G protein-coupled receptors.
The EST sequence of CXCR4 from turbot (Scophthalmus maximus L.) was obtained from a subtractive cDNA library.
In the present study, the full-length cDNA sequence of turbot CXCR4 was obtained, and sequence analysis indicated that its primary structure was highly similar to CXCR4 from other vertebrates.
Quantitative real-time PCR demonstrated that the highest expression level of turbot CXCR4 was in the spleen following injection with physiological saline (PS).
After turbot were challenged with Vibrio harveyi, the lowest expression level of CXCR4 was detected at 8 hours in the spleen and 12 hours in the head kidney, and then increased gradually to 36 hours.
These findings suggested that CXCR4 may play a significant role in the immune response of turbot.
American Psychological Association (APA)
Jia, Airong& Zhang, Xiao-Hua. 2009. Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus. BioMed Research International،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-988442
Modern Language Association (MLA)
Jia, Airong& Zhang, Xiao-Hua. Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus. BioMed Research International No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-988442
American Medical Association (AMA)
Jia, Airong& Zhang, Xiao-Hua. Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus. BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-988442
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988442