High Harvest Yield, High Expansion, and Phenotype Stability of CD146 Mesenchymal Stromal Cells from Whole Primitive Human Umbilical Cord Tissue
Joint Authors
Deasy, Bridget M.
Wescoe, Kristin E.
Schugar, Rebecca C.
Schmidt, Benjamin T.
Nance, Jordan J.
Peault, Bruno
Askew, Yuko
Chirieleison, Steven M.
Evron, Joshua M.
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-11-01
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Human umbilical cord blood is an excellent primitive source of noncontroversial stem cells for treatment of hematologic disorders; meanwhile, new stem cell candidates in the umbilical cord (UC) tissue could provide therapeutic cells for nonhematologic disorders.
We show novel in situ characterization to identify and localize a panel of some markers expressed by mesenchymal stromal cells (MSCs; CD44, CD105, CD73, CD90) and CD146 in the UC.
We describe enzymatic isolation and purification methods of different UC cell populations that do not require manual separation of the vessels and stroma of the coiled, helical-like UC tissue.
Unique quantitation of in situ cell frequency and stromal cell counts upon harvest illustrate the potential to obtain high numerical yields with these methods.
UC stromal cells can differentiate to the osteogenic and chondrogenic lineages and, under specific culturing conditions, they exhibit high expandability with unique long-term stability of their phenotype.
The remarkable stability of the phenotype represents a novel finding for human MSCs, from any source, and supports the use of these cells as highly accessible stromal cells for both basic studies and potentially therapeutic applications such as allogeneic clinical use for musculoskeletal disorders.
American Psychological Association (APA)
Schugar, Rebecca C.& Chirieleison, Steven M.& Wescoe, Kristin E.& Schmidt, Benjamin T.& Askew, Yuko& Nance, Jordan J.…[et al.]. 2009. High Harvest Yield, High Expansion, and Phenotype Stability of CD146 Mesenchymal Stromal Cells from Whole Primitive Human Umbilical Cord Tissue. BioMed Research International،Vol. 2009, no. 2009, pp.1-11.
https://search.emarefa.net/detail/BIM-988446
Modern Language Association (MLA)
Schugar, Rebecca C.…[et al.]. High Harvest Yield, High Expansion, and Phenotype Stability of CD146 Mesenchymal Stromal Cells from Whole Primitive Human Umbilical Cord Tissue. BioMed Research International No. 2009 (2009), pp.1-11.
https://search.emarefa.net/detail/BIM-988446
American Medical Association (AMA)
Schugar, Rebecca C.& Chirieleison, Steven M.& Wescoe, Kristin E.& Schmidt, Benjamin T.& Askew, Yuko& Nance, Jordan J.…[et al.]. High Harvest Yield, High Expansion, and Phenotype Stability of CD146 Mesenchymal Stromal Cells from Whole Primitive Human Umbilical Cord Tissue. BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-11.
https://search.emarefa.net/detail/BIM-988446
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988446