Immunohistochemical and Proteomic Evaluation of Nuclear Ubiquitous Casein and Cyclin-Dependent Kinases Substrate in Invasive Ductal Carcinoma of the Breast
Joint Authors
Zougman, Alexandre
Osiecka, Beata
Ziółkowski, Piotr
Wiśniewski, Jacek R.
Gamian, Elżbieta
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-11-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Nuclear ubiquitous casein and cyclin-dependent kinases substrate (NUCKS) is 27 kDa chromosomal protein of unknown function.
Its amino acid composition as well as structure of its DNA binding domain resembles that of high-mobility group A, HMGA proteins.
HMGA proteins are associated with various malignancies.
Since changes in expression of HMGA are considered as marker of tumor progression, it is possible that similar changes in expression of NUCKS could be useful tool in diagnosis and prognosis of breast cancer.
For identification and analysis of NUCKS we used proteomic and histochemical methods.
Analysis of patient-matched samples of normal and breast cancer by mass spectrometry revealed elevated levels of NUCKS in protein extracts from ductal breast cancers.
We elicited specific antibodies against NUCKS and used them for immunohistochemistry in invasive ductal carcinoma of breast.
We found high expression of NUCKS in 84.3% of cancer cells.
We suggest that such overexpression of NUCKS can play significant role in breast cancer biology.
American Psychological Association (APA)
Ziółkowski, Piotr& Gamian, Elżbieta& Osiecka, Beata& Zougman, Alexandre& Wiśniewski, Jacek R.. 2009. Immunohistochemical and Proteomic Evaluation of Nuclear Ubiquitous Casein and Cyclin-Dependent Kinases Substrate in Invasive Ductal Carcinoma of the Breast. BioMed Research International،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-988477
Modern Language Association (MLA)
Ziółkowski, Piotr…[et al.]. Immunohistochemical and Proteomic Evaluation of Nuclear Ubiquitous Casein and Cyclin-Dependent Kinases Substrate in Invasive Ductal Carcinoma of the Breast. BioMed Research International No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-988477
American Medical Association (AMA)
Ziółkowski, Piotr& Gamian, Elżbieta& Osiecka, Beata& Zougman, Alexandre& Wiśniewski, Jacek R.. Immunohistochemical and Proteomic Evaluation of Nuclear Ubiquitous Casein and Cyclin-Dependent Kinases Substrate in Invasive Ductal Carcinoma of the Breast. BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-988477
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988477