Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector
Joint Authors
Warita, Takayuki
Sakurai, Takayuki
Watanabe, Satoshi
Inoko, Hidetoshi
Ohtsuka, Masato
Sato, Masahiro
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-02-23
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
We developed Cre-loxP-based systems, termed CRTEIL and CETRIZ, which allow gene switching in a noninvasive manner.
Single transfection with pCRTEIL resulted in predominant expression of red fluorescence.
Cotransfection with pCRTEIL and Cre-expression plasmid (pCAG/NCre) caused switching from red to green fluorescence.
Similarly, cotransfection with pCETRIZ and pCAG/NCre resulted in change of green to red fluorescence.
These noninvasive systems will be useful in cell lineage analysis, since descendants of cells exhibiting newly activated gene expression can be continuously monitored in noninvasive fashion.
American Psychological Association (APA)
Ohtsuka, Masato& Warita, Takayuki& Sakurai, Takayuki& Watanabe, Satoshi& Inoko, Hidetoshi& Sato, Masahiro. 2009. Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector. BioMed Research International،Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-988491
Modern Language Association (MLA)
Ohtsuka, Masato…[et al.]. Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector. BioMed Research International No. 2009 (2009), pp.1-9.
https://search.emarefa.net/detail/BIM-988491
American Medical Association (AMA)
Ohtsuka, Masato& Warita, Takayuki& Sakurai, Takayuki& Watanabe, Satoshi& Inoko, Hidetoshi& Sato, Masahiro. Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector. BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-988491
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988491
