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Global Expression Patterns of Three Festuca Species Exposed to Different Doses of Glyphosate Using the Affymetrix GeneChip Wheat Genome Array
Joint Authors
Source
International Journal of Genomics
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-12-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Glyphosate has been shown to act as an inhibitor of an aromatic amino acid biosynthetic pathway, while other pathways that may be affected by glyphosate are not known.
Cross species hybridizations can provide a tool for elucidating biological pathways conserved among organisms.
Comparative genome analyses have indicated a high level of colinearity among grass species and Festuca, on which we focus here, and showed rearrangements common to the Pooideae family.
Based on sequence conservation among grass species, we selected the Affymetrix GeneChip Wheat Genome Array as a tool for the analysis of expression profiles of three Festuca (fescue) species with distinctly different tolerances to varying levels of glyphosate.
Differences in transcript expression were recorded upon foliar glyphosate application at 1.58 mM and 6.32 mM, representing 5% and 20%, respectively, of the recommended rate.
Differences highlighted categories of general metabolic processes, such as photosynthesis, protein synthesis, stress responses, and a larger number of transcripts responded to 20% glyphosate application.
Differential expression of genes encoding proteins involved in the shikimic acid pathway could not be identified by cross hybridization.
Microarray data were confirmed by RT-PCR and qRT-PCR analyses.
This is the first report to analyze the potential of cross species hybridization in Fescue species and the data and analyses will help extend our knowledge on the cellular processes affected by glyphosate.
American Psychological Association (APA)
Cebeci, Ozge& Budak, Hikmet. 2009. Global Expression Patterns of Three Festuca Species Exposed to Different Doses of Glyphosate Using the Affymetrix GeneChip Wheat Genome Array. International Journal of Genomics،Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-988525
Modern Language Association (MLA)
Cebeci, Ozge& Budak, Hikmet. Global Expression Patterns of Three Festuca Species Exposed to Different Doses of Glyphosate Using the Affymetrix GeneChip Wheat Genome Array. International Journal of Genomics No. 2009 (2009), pp.1-10.
https://search.emarefa.net/detail/BIM-988525
American Medical Association (AMA)
Cebeci, Ozge& Budak, Hikmet. Global Expression Patterns of Three Festuca Species Exposed to Different Doses of Glyphosate Using the Affymetrix GeneChip Wheat Genome Array. International Journal of Genomics. 2009. Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-988525
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988525