The Analysis of Multiple Genome Comparisons in Genus Escherichia and Its Application to the Discovery of Uncharacterised Metabolic Genes in Uropathogenic Escherichia coli CFT073
Joint Authors
Zhou, Yuhong
Ward, John M.
Baganz, Frank
Krabben, Preben
Bryant, William A.
Source
International Journal of Genomics
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A survey of a complete gene synteny comparison has been carried out between twenty fully sequenced strains from the genus Escherichia with the aim of finding yet uncharacterised genes implicated in the metabolism of uropathogenic strains of E.
coli (UPEC).
Several sets of adjacent colinear genes have been identified which are present in all four UPEC included in this study (CFT073, F11, UTI89, and 536), annotated with putative metabolic functions, but are not found in any other strains considered.
An operon closely homologous to that encoding the L-sorbose degradation pathway in Klebsiella pneumoniae has been identified in E.
coli CFT073; this operon is present in all of the UPEC considered, but only in 7 of the other 16 strains.
The operon's function has been confirmed by cloning the genes into E.
coli DH5α and testing for growth on L-sorbose.
The functional genomic approach combining in silico and in vitro work presented here can be used as a basis for the discovery of other uncharacterised genes contributing to bacterial survival in specific environments.
American Psychological Association (APA)
Bryant, William A.& Krabben, Preben& Baganz, Frank& Zhou, Yuhong& Ward, John M.. 2010. The Analysis of Multiple Genome Comparisons in Genus Escherichia and Its Application to the Discovery of Uncharacterised Metabolic Genes in Uropathogenic Escherichia coli CFT073. International Journal of Genomics،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-988531
Modern Language Association (MLA)
Bryant, William A.…[et al.]. The Analysis of Multiple Genome Comparisons in Genus Escherichia and Its Application to the Discovery of Uncharacterised Metabolic Genes in Uropathogenic Escherichia coli CFT073. International Journal of Genomics No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-988531
American Medical Association (AMA)
Bryant, William A.& Krabben, Preben& Baganz, Frank& Zhou, Yuhong& Ward, John M.. The Analysis of Multiple Genome Comparisons in Genus Escherichia and Its Application to the Discovery of Uncharacterised Metabolic Genes in Uropathogenic Escherichia coli CFT073. International Journal of Genomics. 2010. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-988531
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988531