![](/images/graphics-bg.png)
Statistical Analysis of Microarray Data with Replicated Spots: A Case Study with Synechococcus WH8102
Joint Authors
Paulsen, I. T.
Thomas, E. V.
Haaland, D. M.
Phillippy, K. H.
Timlin, J. A.
Brahamsha, B.
Elbourne, L. D. H.
Palenik, B.
Source
International Journal of Genomics
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-03-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Until recently microarray experiments often involved relatively few arrays with only a single representation of each gene on each array.
A complete genome microarray with multiple spots per gene (spread out spatially across the array) was developed in order to compare the gene expression of a marine cyanobacterium and a knockout mutant strain in a defined artificial seawater medium.
Statistical methods were developed for analysis in the special situation of this case study where there is gene replication within an array and where relatively few arrays are used, which can be the case with current array technology.
Due in part to the replication within an array, it was possible to detect very small changes in the levels of expression between the wild type and mutant strains.
One interesting biological outcome of this experiment is the indication of the extent to which the phosphorus regulatory system of this cyanobacterium affects the expression of multiple genes beyond those strictly involved in phosphorus acquisition.
American Psychological Association (APA)
Thomas, E. V.& Phillippy, K. H.& Brahamsha, B.& Haaland, D. M.& Timlin, J. A.& Elbourne, L. D. H.…[et al.]. 2009. Statistical Analysis of Microarray Data with Replicated Spots: A Case Study with Synechococcus WH8102. International Journal of Genomics،Vol. 2009, no. 2009, pp.1-11.
https://search.emarefa.net/detail/BIM-988539
Modern Language Association (MLA)
Thomas, E. V.…[et al.]. Statistical Analysis of Microarray Data with Replicated Spots: A Case Study with Synechococcus WH8102. International Journal of Genomics No. 2009 (2009), pp.1-11.
https://search.emarefa.net/detail/BIM-988539
American Medical Association (AMA)
Thomas, E. V.& Phillippy, K. H.& Brahamsha, B.& Haaland, D. M.& Timlin, J. A.& Elbourne, L. D. H.…[et al.]. Statistical Analysis of Microarray Data with Replicated Spots: A Case Study with Synechococcus WH8102. International Journal of Genomics. 2009. Vol. 2009, no. 2009, pp.1-11.
https://search.emarefa.net/detail/BIM-988539
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988539