Sequence Variation and Expression of the Gimap Gene Family in the BB Rat
Joint Authors
Peterson, Morgan R.
Rutledge, Elizabeth A.
Gaur, Prashant
Jensen, Richard
Van Yserloo, Brian
Fuller, Jessica M.
Lernmark, Åke
Ettinger, Ruth A.
Moralejo, Daniel H.
Kwitek, Anne E.
Hoehna, Jana L.
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-03-19
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Positional cloning of lymphopenia (lyp) in the BB rat revealed a frameshift mutation in Gimap5, a member of at least seven related GTPase Immune Associated Protein genes located on rat chromosome 4q24.
Our aim was to clone and sequence the cDNA of the BB diabetes prone (DP) and diabetes resistant (DR) alleles of all seven Gimap genes in the congenic DR.lyp rat line with 2 Mb of BB DP DNA introgressed onto the DR genetic background.
All (100%) DR.lyp/lyp rats are lymphopenic and develop type 1 diabetes (T1D) by 84 days of age while DR.+/+ rats remain T1D and lyp resistant.
Among the seven Gimap genes, the Gimap5 frameshift mutation, a mutant allele that produces no protein, had the greatest impact on lymphopenia in the DR.lyp/lyp rat.
Gimap4 and Gimap1 each had one amino acid substitution of unlikely significance for lymphopenia.
Quantitative RT-PCR analysis showed a reduction in expression of all seven Gimap genes in DR.lyp/lyp spleen and mesenteric lymph nodes when compared to DR.+/+.
Only four; Gimap1, Gimap4, Gimap5, and Gimap9 were reduced in thymus.
Our data substantiates the Gimap5 frameshift mutation as the primary defect with only limited contributions to lymphopenia from the remaining Gimap genes.
American Psychological Association (APA)
Rutledge, Elizabeth A.& Fuller, Jessica M.& Van Yserloo, Brian& Moralejo, Daniel H.& Ettinger, Ruth A.& Gaur, Prashant…[et al.]. 2009. Sequence Variation and Expression of the Gimap Gene Family in the BB Rat. Journal of Diabetes Research،Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-988587
Modern Language Association (MLA)
Rutledge, Elizabeth A.…[et al.]. Sequence Variation and Expression of the Gimap Gene Family in the BB Rat. Journal of Diabetes Research No. 2009 (2009), pp.1-10.
https://search.emarefa.net/detail/BIM-988587
American Medical Association (AMA)
Rutledge, Elizabeth A.& Fuller, Jessica M.& Van Yserloo, Brian& Moralejo, Daniel H.& Ettinger, Ruth A.& Gaur, Prashant…[et al.]. Sequence Variation and Expression of the Gimap Gene Family in the BB Rat. Journal of Diabetes Research. 2009. Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-988587
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988587