Rosiglitazone Prevents High Glucose-Induced Vascular Endothelial Growth Factor and Collagen IV Expression in Cultured Mesangial Cells

Joint Authors

Whiteside, Catharine
Wang, Hong
Xia, Ling
Munk, Snezana
Goldberg, Howard J.
Fantus, I. George

Source

Journal of Diabetes Research

Issue

Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2009-05-17

Country of Publication

Egypt

No. of Pages

11

Main Subjects

Diseases
Medicine

Abstract EN

Peroxisome proliferator-activated receptor (PPARγ), a ligand-dependent transcription factor, negatively modulates high glucose effects.

We postulated that rosiglitazone (RSG), an activator of PPARγ prevents the upregulation of vascular endothelial growth factor (VEGF) and collagen IV by mesangial cells exposed to high glucose.

Primary cultured rat mesangial cells were growth-arrested in 5.6 mM (NG) or 25 mM D-glucose (HG) for up to 48 hours.

In HG, PPARγ mRNA and protein were reduced within 3 h, and enhanced ROS generation, expression of p22phox, VEGF and collagen IV, and PKC-ζ membrane association were prevented by RSG.

In NG, inhibition of PPARγ caused ROS generation and VEGF expression that were unchanged by RSG.

Reduced AMP-activated protein kinase (AMPK) phosphorylation in HG was unchanged with RSG, and VEGF expression was unaffected by AMPK inhibition.

Hence, PPARγ is a negative modulator of HG-induced signaling that acts through PKC-ζ but not AMPK and regulates VEGF and collagen IV expression by mesangial cells.

American Psychological Association (APA)

Whiteside, Catharine& Wang, Hong& Xia, Ling& Munk, Snezana& Goldberg, Howard J.& Fantus, I. George. 2009. Rosiglitazone Prevents High Glucose-Induced Vascular Endothelial Growth Factor and Collagen IV Expression in Cultured Mesangial Cells. Journal of Diabetes Research،Vol. 2009, no. 2009, pp.1-11.
https://search.emarefa.net/detail/BIM-988588

Modern Language Association (MLA)

Whiteside, Catharine…[et al.]. Rosiglitazone Prevents High Glucose-Induced Vascular Endothelial Growth Factor and Collagen IV Expression in Cultured Mesangial Cells. Journal of Diabetes Research No. 2009 (2009), pp.1-11.
https://search.emarefa.net/detail/BIM-988588

American Medical Association (AMA)

Whiteside, Catharine& Wang, Hong& Xia, Ling& Munk, Snezana& Goldberg, Howard J.& Fantus, I. George. Rosiglitazone Prevents High Glucose-Induced Vascular Endothelial Growth Factor and Collagen IV Expression in Cultured Mesangial Cells. Journal of Diabetes Research. 2009. Vol. 2009, no. 2009, pp.1-11.
https://search.emarefa.net/detail/BIM-988588

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-988588