Detection of Autoantibodies against Recombinant Desmoglein 1 and 3 Molecules in Patients with Pemphigus vulgaris: Correlation with Disease Extent at the Time of Diagnosis and during Follow-Up
Joint Authors
Bordignon, Matteo
Peserico, Andrea
Faggion, Diana
Belloni-Fortina, Anna
Pigozzi, Barbara
Alaibac, Mauro
Baldo, Vincenzo
Source
Journal of Immunology Research
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-12-10
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The recent availability of cDNA clones for pemphigus antigens has allowed the production of recombinant desmoglein 1 and desmoglein 3 molecules and the development of an ELISA approach in order to determine levels of antibodies to them.
The aim of the study was to determine the relationship between autoantibodies levels and the extent of both mucosal and skin lesions in 20 patients with pemphigus vulgaris at the time of diagnosis and during follow-up.
For the detection of autoantibodies by ELISA we used the recombinant proteins expressing overlapping sequences with the entire extracellular desmoglein 1 and desmoglein 3 domains.
We showed that in presence of mucosal lesions there was a correlation between extension of mucosal involvement and autoantiboidies titres against both desmoglein 1 and desmoglein 3, whereas in presence of skin lesions there was a statistically significant correlation between extension of skin lesions and autoantibodies titres against desmoglein 3, but not against desmoglein 1.
A not negligible number of patients showed variations of the desmoglein 3 autoantibodies titre which did not correlate with the severity of both cutaneous and mucosal involvement.
Similar results were obtained analyzing autoantibodies titres against desmoglein 1.
In conclusion, we believe that the utilization of recombinant desmoglein 1 and desmoglein 3 proteins by ELISA should be used with caution to monitor disease severity and response to therapy, although it remains a high specific test for the initial diagnosis of pemphigus and the identification of a change in the clinical phenotype of this condition.
American Psychological Association (APA)
Belloni-Fortina, Anna& Faggion, Diana& Pigozzi, Barbara& Peserico, Andrea& Bordignon, Matteo& Baldo, Vincenzo…[et al.]. 2009. Detection of Autoantibodies against Recombinant Desmoglein 1 and 3 Molecules in Patients with Pemphigus vulgaris: Correlation with Disease Extent at the Time of Diagnosis and during Follow-Up. Journal of Immunology Research،Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-988611
Modern Language Association (MLA)
Belloni-Fortina, Anna…[et al.]. Detection of Autoantibodies against Recombinant Desmoglein 1 and 3 Molecules in Patients with Pemphigus vulgaris: Correlation with Disease Extent at the Time of Diagnosis and during Follow-Up. Journal of Immunology Research No. 2009 (2009), pp.1-6.
https://search.emarefa.net/detail/BIM-988611
American Medical Association (AMA)
Belloni-Fortina, Anna& Faggion, Diana& Pigozzi, Barbara& Peserico, Andrea& Bordignon, Matteo& Baldo, Vincenzo…[et al.]. Detection of Autoantibodies against Recombinant Desmoglein 1 and 3 Molecules in Patients with Pemphigus vulgaris: Correlation with Disease Extent at the Time of Diagnosis and during Follow-Up. Journal of Immunology Research. 2009. Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-988611
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988611