The Effect of Calcipotriol on the Expression of Human β Defensin-2 and LL-37 in Cultured Human Keratinocytes
Joint Authors
Hong, Chang Kwun
Lee, Hye In
Kim, Beom Joon
Kim, Myeung Nam
Jeong, Mi Sook
Rho, Yong Kwan
Seo, Seong Jun
Li, Kapsok
Source
Journal of Immunology Research
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-02-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Vitamin D has been reported to regulate innate immunity by controlling the expression of antimicrobial peptides (AMPs).
Objective.
We investigated the effect of calcipotriol on the expression of AMPs in human cultured keratinocytes.
Methods.
Keratinocytes were treated with lipopolysaccharide (LPS), TNF-α, Calcipotriol and irradiated with UVB, cultured, and harvested.
To assess the expression of human beta defensin-2 and LL-37 in the control group, not exposed to any stimulants, the experimental group was treated with LPS, TNF-α, or UVB, and another group was treated again with calcipotriol; reverse transcriptase-polymerase chain reaction, Western blotting, and immunohistochemical staining were performed.
Results.
In the experimental group treated with LPS, UVB irradiation, and TNF-α, the expression of β-defensin and LL-37 was increased more than in the control group and then decreased in the experimental group treated with calcipotriol.
Conclusions.
Calcipotriol suppressed HBD-2 and LL-37, which were stimulated by UVB, LPS, and TNF-α.
American Psychological Association (APA)
Kim, Beom Joon& Rho, Yong Kwan& Lee, Hye In& Jeong, Mi Sook& Li, Kapsok& Seo, Seong Jun…[et al.]. 2010. The Effect of Calcipotriol on the Expression of Human β Defensin-2 and LL-37 in Cultured Human Keratinocytes. Journal of Immunology Research،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-988616
Modern Language Association (MLA)
Kim, Beom Joon…[et al.]. The Effect of Calcipotriol on the Expression of Human β Defensin-2 and LL-37 in Cultured Human Keratinocytes. Journal of Immunology Research No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-988616
American Medical Association (AMA)
Kim, Beom Joon& Rho, Yong Kwan& Lee, Hye In& Jeong, Mi Sook& Li, Kapsok& Seo, Seong Jun…[et al.]. The Effect of Calcipotriol on the Expression of Human β Defensin-2 and LL-37 in Cultured Human Keratinocytes. Journal of Immunology Research. 2010. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-988616
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988616