Immune Reactions Against Elongation Factor 2 Kinase: SpecificPathogenesis of Gastric Ulcer from Helicobacter pylori Infection

Joint Authors

Inaba, Tomoki
Yamamoto, Kazuhide
Oguma, Keiji
Okada, Hiroyuki
Yokota, Kenji
Yamamoto, Yumiko
Ayada, Kiyoshi
Hirai, Kazuyuki
Kita, Masahide
Kawahara, Yoshiro

Source

Journal of Immunology Research

Issue

Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2009-03-24

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Biology

Abstract EN

Helicobacter pylori (H.

pylori) infection is a definite causative factor for gastric ulcers (GUs).

In the present study we detected a specific antigen of gastric epithelial cells (HGC-27) using cell ELISA, which was recognized by the sera of GU patients (n=20) but not in patients with chronic gastritis (CG; n=20) or in healthy volunteers (HC; n=10).

This antigen was over-expressed by a stressful (heat-stressed) environment, and was identified as elongation factor 2 kinase (EF-2K) by western blotting.

The GU patients' lymphocytes stimulated by H.

pylori specifically disrupted heat-stressed HGC-27 cells in a cytotoxic assay.

In flow cytometry, the effector cells (lymphocytes) from GU patients were significantly differentiated to T helper type 1 lymphocyte (Th1) and cytotoxic T lymphocyte (CTL) as opposed to those from CG patients.

The target cells (HGC-27) expressed EF-2K and MHC-class I together with costimulatory molecules from heat stress.

This antigen specific immune mechanism could have a prominent role in the pathogenesis of GU.

American Psychological Association (APA)

Ayada, Kiyoshi& Yokota, Kenji& Kawahara, Yoshiro& Yamamoto, Yumiko& Hirai, Kazuyuki& Inaba, Tomoki…[et al.]. 2009. Immune Reactions Against Elongation Factor 2 Kinase: SpecificPathogenesis of Gastric Ulcer from Helicobacter pylori Infection. Journal of Immunology Research،Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-988622

Modern Language Association (MLA)

Ayada, Kiyoshi…[et al.]. Immune Reactions Against Elongation Factor 2 Kinase: SpecificPathogenesis of Gastric Ulcer from Helicobacter pylori Infection. Journal of Immunology Research No. 2009 (2009), pp.1-10.
https://search.emarefa.net/detail/BIM-988622

American Medical Association (AMA)

Ayada, Kiyoshi& Yokota, Kenji& Kawahara, Yoshiro& Yamamoto, Yumiko& Hirai, Kazuyuki& Inaba, Tomoki…[et al.]. Immune Reactions Against Elongation Factor 2 Kinase: SpecificPathogenesis of Gastric Ulcer from Helicobacter pylori Infection. Journal of Immunology Research. 2009. Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-988622

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-988622