Efficient Isolation of Cardiac Stem Cells from Brown Adipose
Joint Authors
Wang, Changyong
Zhou, Jin
Zhang, Ye
Lin, Qiuxia
Wu, Kuiwu
Wang, Yanmeng
Liu, Zhiqiang
Wang, Haibin
Duan, Cuimi
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Cardiac stem cells represent a logical cell type to exploit in cardiac regeneration.
The efficient harvest of cardiac stem cells from a suitable source would turn promising in cardiac stem cell therapy.
Brown adipose was recently found to be a new source of cardiac stem cells, instrumental to myocardial regeneration.
Unfortunately, an efficient method for the cell isolation is unavailable so far.
In our study we have developed a new method for the efficient isolation of cardiac stem cells from brown adipose by combining different enzymes.
Results showed that the total cell yield dramatically increased (more than 10 times, P<.01) compared with that by previous method.
The content of CD133-positive cells (reported to differentiate into cardiomyocytes with a high frequency) was much higher than that in the previous report (22.43% versus 3.5%).
Moreover, the isolated cells could be the efficiently differentiated into functional cardiomyocytes in optimized conditions.
Thus, the new method we established would be of great use in further exploring cardiac stem cell therapy.
American Psychological Association (APA)
Liu, Zhiqiang& Wang, Haibin& Zhang, Ye& Zhou, Jin& Lin, Qiuxia& Wang, Yanmeng…[et al.]. 2010. Efficient Isolation of Cardiac Stem Cells from Brown Adipose. BioMed Research International،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-988726
Modern Language Association (MLA)
Liu, Zhiqiang…[et al.]. Efficient Isolation of Cardiac Stem Cells from Brown Adipose. BioMed Research International No. 2010 (Dec. 2010), pp.1-9.
https://search.emarefa.net/detail/BIM-988726
American Medical Association (AMA)
Liu, Zhiqiang& Wang, Haibin& Zhang, Ye& Zhou, Jin& Lin, Qiuxia& Wang, Yanmeng…[et al.]. Efficient Isolation of Cardiac Stem Cells from Brown Adipose. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-988726
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-988726